6ksn: Difference between revisions
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New page: '''Unreleased structure''' The entry 6ksn is ON HOLD until Paper Publication Authors: Kumar, S., Athreya, A., Penmatsa, A. Description: Structure of a Zn-bound camelid single domain an... |
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==Structure of a Zn-bound camelid single domain antibody== | |||
<StructureSection load='6ksn' size='340' side='right'caption='[[6ksn]], [[Resolution|resolution]] 2.15Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6ksn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KSN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KSN FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ksn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ksn OCA], [https://pdbe.org/6ksn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ksn RCSB], [https://www.ebi.ac.uk/pdbsum/6ksn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ksn ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Single chain antibodies from camelids have served as powerful tools ranging from diagnostics and therapeutics to crystallization chaperones meant to study protein structure and function. In this study, we isolated a single chain antibody from an Indian dromedary camel (ICab) immunized against a bacterial 14TM helix transporter, NorC, from Staphylococcus aureus We identified this antibody in a yeast display screen built from mononuclear cells isolated from the immunized camel and purified the antibody from E.coli after refolding it from inclusion bodies. The X-ray structure of the antibody at 2.15 A resolution revealed a unique feature within its CDR3 loop, which harbors a Zn(2+)-binding site that substitutes for a loop-stabilizing disulfide bond. We performed mutagenesis to compromise the Zn(2+)-binding site and observed that this change severely hampered antibody stability and its ability to interact with the antigen. The lack of bound Zn(2+)also made the CDR3 loop highly flexible as observed in all-atom simulations. Using confocal imaging of NorC-expressing E.coli spheroplasts, we found that the ICab interacts with the extracellular surface of NorC. This suggests that the ICab could be a valuable tool for detecting methicillin-resistant S.aureus (MRSA) strains that express efflux transporters such as NorC in hospital and community settings. | |||
Isolation and structural characterization of a Zn(2+)-bound single-domain antibody against NorC, a putative multi-drug efflux transporter in bacteria.,Kumar S, Ithayaraja M, Athreya A, Ranjan R, Penmatsa A J Biol Chem. 2019 Nov 7. pii: RA119.010902. doi: 10.1074/jbc.RA119.010902. PMID:31699895<ref>PMID:31699895</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Athreya | <div class="pdbe-citations 6ksn" style="background-color:#fffaf0;"></div> | ||
[[Category: Kumar | == References == | ||
[[Category: Penmatsa | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Camelus dromedarius]] | |||
[[Category: Large Structures]] | |||
[[Category: Athreya A]] | |||
[[Category: Kumar S]] | |||
[[Category: Penmatsa A]] | |||
Latest revision as of 10:42, 22 November 2023
Structure of a Zn-bound camelid single domain antibody
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