6kx9: Difference between revisions

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~~'''Unreleased structure'''~~

~~The entry~~ 6kx9 is ~~ON HOLD~~

==Crystal structure of 8-mer peptide from avian influenza H5N1 virus in complex with BF2*1501==

== Structural highlights ==

<table><tr><td colspan='2'>[[6kx9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KX9 FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.902Å</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kx9 OCA], [https://pdbe.org/6kx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kx9 RCSB], [https://www.ebi.ac.uk/pdbsum/6kx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kx9 ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/Q9GIP6_CHICK Q9GIP6_CHICK]

== Publication Abstract from PubMed ==

Lethal infections by strains of the highly-pathogenic avian influenza virus (HPAIV) H5N1 pose serious threats to both the poultry industry and public health worldwide. A lack of confirmed HPAIV epitopes recognized by cytotoxic T lymphocytes (CTLs) has hindered the utilization of CD8(+) T-cell-mediated immunity and has precluded the development of effectively diversified epitope-based vaccination approaches. In particular, an HPAIV H5N1 CTL-recognized epitope based on the peptide MHC-I-beta2m (pMHC-I) complex has not yet been designed. Here, screening a collection of selected peptides of several HPAIV strains against a specific pathogen-free pMHC-I (pBF2*1501), we identified a highly-conserved HPAIV H5N1 CTL epitope, named HPAIV-PA123-130 We determined the structure of the BF2*1501-PA123-130 complex at 2.1 A resolution to elucidate the molecular mechanisms of a preferential presentation of the highly-conserved PA123-130 epitope in the chicken B15 lineage. Conformational characteristics of the PA123-130 epitope with a protruding Tyr-7 residue indicated that this epitope has great potential to be recognized by specific TCRs. Moreover, significantly increased numbers of CD8(+) T cells specific for the HPAIV-PA123-130 epitope in peptide-immunized chickens indicated that a repertoire of CD8(+) T cells can specifically respond to this epitope. We anticipate that the identification and structural characterization of the PA123-130 epitope reported here could enable further studies of CTL immunity against HPAIV H5N1. Such studies may aid in the development of vaccine development strategies using well-conserved internal viral antigens in chickens.

~~Authors~~:

Structures of the MHC-I molecule BF2*1501 disclose the preferred presentation of an H5N1 virus-derived epitope.,Li X, Zhang L, Liu Y, Ma L, Zhang N, Xia C J Biol Chem. 2020 Apr 17;295(16):5292-5306. doi: 10.1074/jbc.RA120.012713. Epub, 2020 Mar 9. PMID:32152225<ref>PMID:32152225</ref>

~~Description~~:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased~~ Structures]]

</div>

==See Also==

*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]

*[[MHC 3D structures|MHC 3D structures]]

*[[MHC I 3D structures|MHC I 3D structures]]

== References ==

__TOC__

</StructureSection>

[[Category: Gallus gallus]]

[[Category: Large Structures]]

[[Category: Synthetic construct]]

[[Category: Xiao L]]

[[Category: Zhang L]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6kx9 is ON HOLD
+==Crystal structure of 8-mer peptide from avian influenza H5N1 virus in complex with BF2*1501==
+<StructureSection load='6kx9' size='340' side='right'caption='[[6kx9]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6kx9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KX9 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.902&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kx9 OCA], [https://pdbe.org/6kx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kx9 RCSB], [https://www.ebi.ac.uk/pdbsum/6kx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kx9 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9GIP6_CHICK Q9GIP6_CHICK]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Lethal infections by strains of the highly-pathogenic avian influenza virus (HPAIV) H5N1 pose serious threats to both the poultry industry and public health worldwide. A lack of confirmed HPAIV epitopes recognized by cytotoxic T lymphocytes (CTLs) has hindered the utilization of CD8(+) T-cell-mediated immunity and has precluded the development of effectively diversified epitope-based vaccination approaches. In particular, an HPAIV H5N1 CTL-recognized epitope based on the peptide MHC-I-beta2m (pMHC-I) complex has not yet been designed. Here, screening a collection of selected peptides of several HPAIV strains against a specific pathogen-free pMHC-I (pBF2*1501), we identified a highly-conserved HPAIV H5N1 CTL epitope, named HPAIV-PA123-130 We determined the structure of the BF2*1501-PA123-130 complex at 2.1 A resolution to elucidate the molecular mechanisms of a preferential presentation of the highly-conserved PA123-130 epitope in the chicken B15 lineage. Conformational characteristics of the PA123-130 epitope with a protruding Tyr-7 residue indicated that this epitope has great potential to be recognized by specific TCRs. Moreover, significantly increased numbers of CD8(+) T cells specific for the HPAIV-PA123-130 epitope in peptide-immunized chickens indicated that a repertoire of CD8(+) T cells can specifically respond to this epitope. We anticipate that the identification and structural characterization of the PA123-130 epitope reported here could enable further studies of CTL immunity against HPAIV H5N1. Such studies may aid in the development of vaccine development strategies using well-conserved internal viral antigens in chickens.
-Authors:
+Structures of the MHC-I molecule BF2*1501 disclose the preferred presentation of an H5N1 virus-derived epitope.,Li X, Zhang L, Liu Y, Ma L, Zhang N, Xia C J Biol Chem. 2020 Apr 17;295(16):5292-5306. doi: 10.1074/jbc.RA120.012713. Epub, 2020 Mar 9. PMID:32152225<ref>PMID:32152225</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6kx9" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
+*[[MHC 3D structures|MHC 3D structures]]
+*[[MHC I 3D structures|MHC I 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Gallus gallus]]
+[[Category: Large Structures]]
+[[Category: Synthetic construct]]
+[[Category: Xiao L]]
+[[Category: Zhang L]]