6snl: Difference between revisions
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==(R)-selective amine transaminase from Exophiala sideris== | |||
<StructureSection load='6snl' size='340' side='right'caption='[[6snl]], [[Resolution|resolution]] 3.13Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6snl]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Exophiala_sideris Exophiala sideris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SNL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SNL FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.129Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6snl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6snl OCA], [https://pdbe.org/6snl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6snl RCSB], [https://www.ebi.ac.uk/pdbsum/6snl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6snl ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0D1XFW6_9EURO A0A0D1XFW6_9EURO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Amine transaminases (ATAs) are used to synthesize enantiomerically pure amines, which are building blocks for pharmaceuticals and agrochemicals. (R) -selective ATAs belong to the fold type IV PLP dependent enzymes and different sequence-, structure- and substrate scope-based features have been identified in the past decade. However, our knowledge is still restricted due to the limited number of characterized (R) -ATAs with additional bias towards fungal origin. We aimed to expand the toolbox of (R) -ATAs and contribute to the understanding of this enzyme subfamily. We identified and characterized four new (R) -ATAs. The ATA from Exophiala sideris contains a motif characteristic for D-ATAs, which was previously believed to be a disqualifying factor for (R) -ATA activity. The crystal structure of the ATA from Shinella is the first from a gram-negative bacterium. The ATAs from Pseudonocardia acaciae and Tetrasphaera japonica are the first characterized (R) -ATAs with a shortened/missing N-terminal helix. The active site charges vary significantly between the new and known ATAs correlating with their diverging substrate scope. | |||
Expanding the Toolbox of (R)-selective Amine Transaminases by Identification and Characterization of new Members.,Telzerow A, Paris J, Hakansson M, Gonzalez-Sabin J, Rios-Lombardia N, Groger H, Moris F, Schurmann M, Schwab H, Steiner K Chembiochem. 2020 Nov 26. doi: 10.1002/cbic.202000692. PMID:33242357<ref>PMID:33242357</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6snl" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Exophiala sideris]] | |||
[[Category: Large Structures]] | |||
[[Category: Hakansson M]] | |||
[[Category: Steiner K]] | |||
[[Category: Telzerow A]] | |||
Latest revision as of 12:44, 24 January 2024
(R)-selective amine transaminase from Exophiala sideris
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