6srq: Difference between revisions
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New page: '''Unreleased structure''' The entry 6srq is ON HOLD Authors: Kloos, M., Gorel, A., Nass, K. Description: X-ray pump X-ray probe on thaumatin nanocrystals: 18 fs time delay [[Category:... |
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The | ==X-ray pump X-ray probe on thaumatin nanocrystals: 18 fs time delay== | ||
<StructureSection load='6srq' size='340' side='right'caption='[[6srq]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SRQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SRQ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6srq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6srq OCA], [https://pdbe.org/6srq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6srq RCSB], [https://www.ebi.ac.uk/pdbsum/6srq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6srq ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
X-ray free-electron lasers (XFELs) enable crystallographic structure determination beyond the limitations imposed upon synchrotron measurements by radiation damage. The need for very short XFEL pulses is relieved through gating of Bragg diffraction by loss of crystalline order as damage progresses, but not if ionization events are spatially non-uniform due to underlying elemental distributions, as in biological samples. Indeed, correlated movements of iron and sulfur ions were observed in XFEL-irradiated ferredoxin microcrystals using unusually long pulses of 80 fs. Here, we report a femtosecond time-resolved X-ray pump/X-ray probe experiment on protein nanocrystals. We observe changes in the protein backbone and aromatic residues as well as disulfide bridges. Simulations show that the latter's correlated structural dynamics are much slower than expected for the predicted high atomic charge states due to significant impact of ion caging and plasma electron screening. This indicates that dense-environment effects can strongly affect local radiation damage-induced structural dynamics. | |||
Structural dynamics in proteins induced by and probed with X-ray free-electron laser pulses.,Nass K, Gorel A, Abdullah MM, V Martin A, Kloos M, Marinelli A, Aquila A, Barends TRM, Decker FJ, Bruce Doak R, Foucar L, Hartmann E, Hilpert M, Hunter MS, Jurek Z, Koglin JE, Kozlov A, Lutman AA, Kovacs GN, Roome CM, Shoeman RL, Santra R, Quiney HM, Ziaja B, Boutet S, Schlichting I Nat Commun. 2020 Apr 14;11(1):1814. doi: 10.1038/s41467-020-15610-4. PMID:32286284<ref>PMID:32286284</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Gorel | <div class="pdbe-citations 6srq" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Gorel A]] | |||
[[Category: Kloos M]] | |||
[[Category: Nass K]] | |||
Latest revision as of 13:07, 6 November 2024
X-ray pump X-ray probe on thaumatin nanocrystals: 18 fs time delay
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