5i2t: Difference between revisions

Latest revision as of 08:21, 23 August 2023

Domain characterization of the WD protein Pwp2 and their relevance in ribosome biogenesis

Structural highlights

5i2t is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.543Å
Ligands:	SO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PWP2_YEAST Required for bud-site selection and cell separation. Also involved in nucleolar processing of pre-18S ribosomal RNA.^[1] ^[2]

Publication Abstract from PubMed

The SSU processome constitutes a large ribonucleoprotein complex involved in the early steps of ribosome biogenesis. UTP-B is one of the first multi-subunit protein complexes that associates with the pre-ribosomal RNA to form the SSU processome. To understand the molecular basis of the hierarchical assembly of the SSU-processome, we have undergone a structural and functional analysis of the UTP-B subunit Pwp2p. We show that Pwp2p is required for the proper assembly of UTP-B and for a productive association of UTP-B with pre-rRNA. These two functions are mediated by two distinct structural domains. The N-terminal domain of Pwp2p folds into a tandem WD-repeat (tWD) that associates with Utp21p, Utp18p, and Utp6p to form a core complex. The CTDs of Pwp2p and Utp21p mediate the assembly of the heterodimer Utp12p:Utp13p that is required for the stable incorporation of the UTP-B complex in the SSU processome. Finally, we provide evidence suggesting a role of UTP-B as a platform for the binding of assembly factors during the maturation of 20S rRNA precursors.

Pwp2 mediates UTP-B assembly via two structurally independent domains.,Boissier F, Schmidt CM, Linnemann J, Fribourg S, Perez-Fernandez J Sci Rep. 2017 Jun 9;7(1):3169. doi: 10.1038/s41598-017-03034-y. PMID:28600509^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dragon F, Gallagher JE, Compagnone-Post PA, Mitchell BM, Porwancher KA, Wehner KA, Wormsley S, Settlage RE, Shabanowitz J, Osheim Y, Beyer AL, Hunt DF, Baserga SJ. A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature. 2002 Jun 27;417(6892):967-70. Epub 2002 Jun 9. PMID:12068309 doi:https://dx.doi.org/10.1038/nature00769
↑ Shafaatian R, Payton MA, Reid JD. PWP2, a member of the WD-repeat family of proteins, is an essential Saccharomyces cerevisiae gene involved in cell separation. Mol Gen Genet. 1996 Aug 27;252(1-2):101-14. PMID:8804409
↑ Boissier F, Schmidt CM, Linnemann J, Fribourg S, Perez-Fernandez J. Pwp2 mediates UTP-B assembly via two structurally independent domains. Sci Rep. 2017 Jun 9;7(1):3169. doi: 10.1038/s41598-017-03034-y. PMID:28600509 doi:https://dx.doi.org/10.1038/s41598-017-03034-y

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Dragon F, Gallagher JE, Compagnone-Post PA, Mitchell BM, Porwancher KA, Wehner KA, Wormsley S, Settlage RE, Shabanowitz J, Osheim Y, Beyer AL, Hunt DF, Baserga SJ. A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis. Nature. 2002 Jun 27;417(6892):967-70. Epub 2002 Jun 9. PMID:12068309 doi:https://dx.doi.org/10.1038/nature00769

[2] Shafaatian R, Payton MA, Reid JD. PWP2, a member of the WD-repeat family of proteins, is an essential Saccharomyces cerevisiae gene involved in cell separation. Mol Gen Genet. 1996 Aug 27;252(1-2):101-14. PMID:8804409

[3] Boissier F, Schmidt CM, Linnemann J, Fribourg S, Perez-Fernandez J. Pwp2 mediates UTP-B assembly via two structurally independent domains. Sci Rep. 2017 Jun 9;7(1):3169. doi: 10.1038/s41598-017-03034-y. PMID:28600509 doi:https://dx.doi.org/10.1038/s41598-017-03034-y

[1]

[2]

[3]

@@ Line 3: / Line 3: @@
 <StructureSection load='5i2t' size='340' side='right'caption='[[5i2t]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5i2t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I2T FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5i2t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I2T FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.543&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PWP2, UTP1, YCR057C, YCR55C/YCR57C/YCR58C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i2t OCA], [http://pdbe.org/5i2t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i2t RCSB], [http://www.ebi.ac.uk/pdbsum/5i2t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i2t ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i2t OCA], [https://pdbe.org/5i2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i2t RCSB], [https://www.ebi.ac.uk/pdbsum/5i2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i2t ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PWP2_YEAST PWP2_YEAST]] Required for bud-site selection and cell separation. Also involved in nucleolar processing of pre-18S ribosomal RNA.<ref>PMID:12068309</ref> <ref>PMID:8804409</ref>
+[https://www.uniprot.org/uniprot/PWP2_YEAST PWP2_YEAST] Required for bud-site selection and cell separation. Also involved in nucleolar processing of pre-18S ribosomal RNA.<ref>PMID:12068309</ref> <ref>PMID:8804409</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 18824]]
 [[Category: Large Structures]]
-[[Category: Boissier, F]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Fribourg, S]]
+[[Category: Boissier F]]
-[[Category: Biosynthetic protein]]
+[[Category: Fribourg S]]
-[[Category: Ribosome biogenesis]]
-[[Category: Wd]]

5i2t: Difference between revisions

Latest revision as of 08:21, 23 August 2023

Domain characterization of the WD protein Pwp2 and their relevance in ribosome biogenesis

Structural highlights

Function

Publication Abstract from PubMed

References

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