2y9k: Difference between revisions

Latest revision as of 18:18, 18 October 2023

Three-dimensional model of Salmonella's needle complex at subnanometer resolution

Loading...

Status: waiting for Mol* initialization

Structural highlights

2y9k is a 15 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 8.3Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SCTC1_SALTY Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:9786184, PubMed:27974800, PubMed:30242280). Forms a ring-shaped multimeric structure with an apparent central pore in the outer membrane (PubMed:9786184, PubMed:21385715, PubMed:27974800, PubMed:30242280).^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

Type III secretion systems (T3SSs) are essential virulence factors used by many Gram-negative bacteria to inject proteins that make eukaryotic host cells accessible to invasion. The T3SS core structure, the needle complex (NC), is a ~3.5 megadalton-sized, oligomeric, membrane-embedded complex. Analyzing cryo-electron microscopy images of top views of NCs or NC substructures from Salmonella typhimurium revealed a 24-fold symmetry for the inner rings and a 15-fold symmetry for the outer rings, giving an overall C3 symmetry. Local refinement and averaging showed the organization of the central core and allowed us to reconstruct a subnanometer composite structure of the NC, which together with confident docking of atomic structures reveal insights into its overall organization and structural requirements during assembly.

Three-dimensional model of Salmonella's needle complex at subnanometer resolution.,Schraidt O, Marlovits TC Science. 2011 Mar 4;331(6021):1192-5. PMID:21385715^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schraidt O, Marlovits TC. Three-dimensional model of Salmonella's needle complex at subnanometer resolution. Science. 2011 Mar 4;331(6021):1192-5. PMID:21385715 doi:10.1126/science.1199358
↑ Worrall LJ, Hong C, Vuckovic M, Deng W, Bergeron JR, Majewski DD, Huang RK, Spreter T, Finlay BB, Yu Z, Strynadka NC. Near-atomic-resolution cryo-EM analysis of the Salmonella T3S injectisome basal body. Nature. 2016 Dec 14. doi: 10.1038/nature20576. PMID:27974800 doi:https://dx.doi.org/10.1038/nature20576
↑ Hu J, Worrall LJ, Hong C, Vuckovic M, Atkinson CE, Caveney N, Yu Z, Strynadka NCJ. Cryo-EM analysis of the T3S injectisome reveals the structure of the needle and open secretin. Nat Commun. 2018 Sep 21;9(1):3840. doi: 10.1038/s41467-018-06298-8. PMID:30242280 doi:https://dx.doi.org/10.1038/s41467-018-06298-8
↑ Crago AM, Koronakis V. Salmonella InvG forms a ring-like multimer that requires the InvH lipoprotein for outer membrane localization. Mol Microbiol. 1998 Oct;30(1):47-56. doi: 10.1046/j.1365-2958.1998.01036.x. PMID:9786184 doi:https://dx.doi.org/10.1046/j.1365-2958.1998.01036.x
↑ Schraidt O, Marlovits TC. Three-dimensional model of Salmonella's needle complex at subnanometer resolution. Science. 2011 Mar 4;331(6021):1192-5. PMID:21385715 doi:10.1126/science.1199358

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Schraidt O, Marlovits TC. Three-dimensional model of Salmonella's needle complex at subnanometer resolution. Science. 2011 Mar 4;331(6021):1192-5. PMID:21385715 doi:10.1126/science.1199358

[2] Worrall LJ, Hong C, Vuckovic M, Deng W, Bergeron JR, Majewski DD, Huang RK, Spreter T, Finlay BB, Yu Z, Strynadka NC. Near-atomic-resolution cryo-EM analysis of the Salmonella T3S injectisome basal body. Nature. 2016 Dec 14. doi: 10.1038/nature20576. PMID:27974800 doi:https://dx.doi.org/10.1038/nature20576

[3] Hu J, Worrall LJ, Hong C, Vuckovic M, Atkinson CE, Caveney N, Yu Z, Strynadka NCJ. Cryo-EM analysis of the T3S injectisome reveals the structure of the needle and open secretin. Nat Commun. 2018 Sep 21;9(1):3840. doi: 10.1038/s41467-018-06298-8. PMID:30242280 doi:https://dx.doi.org/10.1038/s41467-018-06298-8

[4] Crago AM, Koronakis V. Salmonella InvG forms a ring-like multimer that requires the InvH lipoprotein for outer membrane localization. Mol Microbiol. 1998 Oct;30(1):47-56. doi: 10.1046/j.1365-2958.1998.01036.x. PMID:9786184 doi:https://dx.doi.org/10.1046/j.1365-2958.1998.01036.x

[5] Schraidt O, Marlovits TC. Three-dimensional model of Salmonella's needle complex at subnanometer resolution. Science. 2011 Mar 4;331(6021):1192-5. PMID:21385715 doi:10.1126/science.1199358

[1]

[2]

[3]

[4]

[5]

@@ Line 1: / Line 1: @@
 ==Three-dimensional model of Salmonella's needle complex at subnanometer resolution==
-<StructureSection load='2y9k' size='340' side='right'caption='[[2y9k]], [[Resolution|resolution]] 8.30&Aring;' scene=''>
+<SX load='2y9k' size='340' side='right' viewer='molstar' caption='[[2y9k]], [[Resolution|resolution]] 8.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2y9k]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y9K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2Y9K FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2y9k]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y9K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y9K FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2y9j|2y9j]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.3&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y9k OCA], [http://pdbe.org/2y9k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2y9k RCSB], [http://www.ebi.ac.uk/pdbsum/2y9k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2y9k ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y9k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y9k OCA], [https://pdbe.org/2y9k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y9k RCSB], [https://www.ebi.ac.uk/pdbsum/2y9k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y9k ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/INVG_SALTY INVG_SALTY]] Involved in the invasion of the cells of the intestinal epithelium. Could be necessary for the export of invasion related determinants.
+[https://www.uniprot.org/uniprot/SCTC1_SALTY SCTC1_SALTY] Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:9786184, PubMed:27974800, PubMed:30242280). Forms a ring-shaped multimeric structure with an apparent central pore in the outer membrane (PubMed:9786184, PubMed:21385715, PubMed:27974800, PubMed:30242280).<ref>PMID:21385715</ref> <ref>PMID:27974800</ref> <ref>PMID:30242280</ref> <ref>PMID:9786184</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 21: @@
 <references/>
 __TOC__
-</StructureSection>
+</SX>
 [[Category: Large Structures]]
-[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
+[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
-[[Category: Marlovits, T C]]
+[[Category: Marlovits TC]]
-[[Category: Schraidt, O]]
+[[Category: Schraidt O]]
-[[Category: C15 fold]]
-[[Category: Outer membrane ring]]
-[[Category: Protein transport]]
-[[Category: Secretin family]]
-[[Category: Type iii secretion system]]

2y9k: Difference between revisions

Latest revision as of 18:18, 18 October 2023

Three-dimensional model of Salmonella's needle complex at subnanometer resolution

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools