4b5y: Difference between revisions

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<StructureSection load='4b5y' size='340' side='right'caption='[[4b5y]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
<StructureSection load='4b5y' size='340' side='right'caption='[[4b5y]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4b5y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B5Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B5Y FirstGlance]. <br>
<table><tr><td colspan='2'>[[4b5y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B5Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B5Y FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SWG:2-[(4Z)-2-[(1R)-1-AMINO-2-HYDROXY-ETHYL]-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-IMIDAZOL-1-YL]ETHANOIC+ACID'>SWG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1b9c|1b9c]], [[1bfp|1bfp]], [[1c4f|1c4f]], [[1cv7|1cv7]], [[1ema|1ema]], [[1emb|1emb]], [[1emc|1emc]], [[1eme|1eme]], [[1emf|1emf]], [[1emg|1emg]], [[1emk|1emk]], [[1eml|1eml]], [[1emm|1emm]], [[1f09|1f09]], [[1f0b|1f0b]], [[1gfl|1gfl]], [[1h6r|1h6r]], [[1hcj|1hcj]], [[1huy|1huy]], [[1jby|1jby]], [[1jbz|1jbz]], [[1jc0|1jc0]], [[1jc1|1jc1]], [[1kp5|1kp5]], [[1kyp|1kyp]], [[1kyr|1kyr]], [[1kys|1kys]], [[1myw|1myw]], [[1q4a|1q4a]], [[1q4b|1q4b]], [[1q4c|1q4c]], [[1q4d|1q4d]], [[1q4e|1q4e]], [[1q73|1q73]], [[1qxt|1qxt]], [[1qy3|1qy3]], [[1qyf|1qyf]], [[1qyo|1qyo]], [[1qyq|1qyq]], [[1rm9|1rm9]], [[1rmm|1rmm]], [[1rmo|1rmo]], [[1rmp|1rmp]], [[1rrx|1rrx]], [[1w7s|1w7s]], [[1w7t|1w7t]], [[1w7u|1w7u]], [[1yfp|1yfp]], [[1yhg|1yhg]], [[1yhh|1yhh]], [[1yhi|1yhi]], [[1yj2|1yj2]], [[1yjf|1yjf]], [[1z1p|1z1p]], [[1z1q|1z1q]], [[2ah8|2ah8]], [[2aha|2aha]], [[2b3p|2b3p]], [[2b3q|2b3q]], [[2emd|2emd]], [[2emn|2emn]], [[2emo|2emo]], [[2fwq|2fwq]], [[2fzu|2fzu]], [[2wsn|2wsn]], [[2wso|2wso]], [[2wur|2wur]], [[2y0g|2y0g]], [[2ydz|2ydz]], [[2ye0|2ye0]], [[2ye1|2ye1]], [[2yfp|2yfp]], [[3ztf|3ztf]], [[4anj|4anj]], [[4ar7|4ar7]], [[4as8|4as8]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SWG:2-[(4Z)-2-[(1R)-1-AMINO-2-HYDROXY-ETHYL]-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-IMIDAZOL-1-YL]ETHANOIC+ACID'>SWG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b5y OCA], [http://pdbe.org/4b5y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4b5y RCSB], [http://www.ebi.ac.uk/pdbsum/4b5y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4b5y ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b5y OCA], [https://pdbe.org/4b5y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b5y RCSB], [https://www.ebi.ac.uk/pdbsum/4b5y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b5y ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.  
[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Optimization of autofluorescent proteins by intensity-based screening of bacteria does not necessarily identify the brightest variant for eukaryotes. We report a strategy to screen excited state lifetimes, which identified cyan fluorescent proteins with long fluorescence lifetimes (&gt;3.7 ns) and high quantum yields (&gt;0.8). One variant, mTurquoise, was 1.5-fold brighter than mCerulean in mammalian cells and decayed mono-exponentially, making it an excellent fluorescence resonance energy transfer (FRET) donor.
 
Bright cyan fluorescent protein variants identified by fluorescence lifetime screening.,Goedhart J, van Weeren L, Hink MA, Vischer NO, Jalink K, Gadella TW Jr Nat Methods. 2010 Feb;7(2):137-9. Epub 2010 Jan 17. PMID:20081836<ref>PMID:20081836</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4b5y" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Aeqvi]]
[[Category: Aequorea victoria]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Gadella, T W.J]]
[[Category: Gadella TWJ]]
[[Category: Goedhart, J]]
[[Category: Goedhart J]]
[[Category: Lelimousin, M]]
[[Category: Lelimousin M]]
[[Category: Noirclerc-Savoye, M]]
[[Category: Noirclerc-Savoye M]]
[[Category: Oost, K]]
[[Category: Oost K]]
[[Category: Royant, A]]
[[Category: Royant A]]
[[Category: Stetten, D von]]
[[Category: Von Stetten D]]
[[Category: Cfp]]
[[Category: Fluorescent protein]]
[[Category: Fret]]
[[Category: Fret donor]]
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