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[[Image:3bvq.jpg|left|200px]]
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{{STRUCTURE_3bvq|  PDB=3bvq  |  SCENE=  }}
'''Crystal Structure of Apo NotI Restriction Endonuclease'''


==Crystal Structure of Apo NotI Restriction Endonuclease==
<StructureSection load='3bvq' size='340' side='right'caption='[[3bvq]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3bvq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nocardia_otitidiscaviarum Nocardia otitidiscaviarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BVQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bvq OCA], [https://pdbe.org/3bvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bvq RCSB], [https://www.ebi.ac.uk/pdbsum/3bvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bvq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q2I6W2_9NOCA Q2I6W2_9NOCA]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of the rare-cutting restriction endonuclease NotI, which recognizes the 8 bp target 5'-GCGGCCGC-3', has been solved with and without bound DNA. Because of its specificity (recognizing a site that occurs once per 65 kb), NotI is used to generate large genomic fragments and to map DNA methylation status. NotI contains a unique metal binding fold, found in a variety of putative endonucleases, occupied by an iron atom coordinated within a tetrahedral Cys4 motif. This domain positions nearby protein elements for DNA recognition, and serves a structural role. While recognition of the central six base pairs of the target is accomplished via a saturated hydrogen bond network typical of restriction enzymes, the most peripheral base pairs are engaged in a single direct contact in the major groove, reflecting reduced pressure to recognize those positions. NotI may represent an evolutionary intermediate between mobile endonucleases (which recognize longer target sites) and canonical restriction endonucleases.


==Overview==
Structures of the Rare-Cutting Restriction Endonuclease NotI Reveal a Unique Metal Binding Fold Involved in DNA Binding.,Lambert AR, Sussman D, Shen B, Maunus R, Nix J, Samuelson J, Xu SY, Stoddard BL Structure. 2008 Apr;16(4):558-69. PMID:18400177<ref>PMID:18400177</ref>
The structure of the rare-cutting restriction endonuclease NotI, which recognizes the 8 bp target 5'-GCGGCCGC-3', has been solved with and without bound DNA. Because of its specificity (recognizing a site that occurs once per 65 kb), NotI is used to generate large genomic fragments and to map DNA methylation status. NotI contains a unique metal binding fold, found in a variety of putative endonucleases, occupied by an iron atom coordinated within a tetrahedral Cys4 motif. This domain positions nearby protein elements for DNA recognition, and serves a structural role. While recognition of the central six base pairs of the target is accomplished via a saturated hydrogen bond network typical of restriction enzymes, the most peripheral base pairs are engaged in a single direct contact in the major groove, reflecting reduced pressure to recognize those positions. NotI may represent an evolutionary intermediate between mobile endonucleases (which recognize longer target sites) and canonical restriction endonucleases.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
3BVQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nocardia_otitidiscaviarum Nocardia otitidiscaviarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BVQ OCA].
</div>
<div class="pdbe-citations 3bvq" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structures of the Rare-Cutting Restriction Endonuclease NotI Reveal a Unique Metal Binding Fold Involved in DNA Binding., Lambert AR, Sussman D, Shen B, Maunus R, Nix J, Samuelson J, Xu SY, Stoddard BL, Structure. 2008 Apr;16(4):558-69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18400177 18400177]
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Nocardia otitidiscaviarum]]
[[Category: Nocardia otitidiscaviarum]]
[[Category: Single protein]]
[[Category: Lambert AR]]
[[Category: Lambert, A R.]]
[[Category: Shen B]]
[[Category: Shen, B.]]
[[Category: Stoddard BL]]
[[Category: Stoddard, B L.]]
[[Category: Sussman D]]
[[Category: Sussman, D.]]
[[Category: Fe-cys4 motif]]
[[Category: Hydrolase]]
[[Category: Iron-sulfur protein]]
[[Category: Rare-cutting]]
[[Category: Restriction endonuclease]]
[[Category: Restriction enzyme fold]]
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