1fof: Difference between revisions

Latest revision as of 07:16, 9 October 2024

CRYSTAL STRUCTURE OF THE CLASS D BETA-LACTAMASE OXA-10

Structural highlights

1fof is a 2 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:	CO, SO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BLO10_PSEAI Hydrolyzes both carbenicillin and oxacillin.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We report the crystal structure of a class D beta-lactamase, the broad spectrum enzyme OXA-10 from Pseudomonas aeruginosa at 2.0 A resolution. There are significant differences between the overall fold observed in this structure and those of the evolutionarily related class A and class C beta-lactamases. Furthermore, the structure suggests the unique, cation mediated formation of a homodimer. Kinetic and hydrodynamic data shows that the dimer is a relevant species in solution and is the more active form of the enzyme. Comparison of the molecular details of the active sites of the class A and class C enzymes with the OXA-10 structure reveals that there is no counterpart in OXA-10 to the residues proposed to act as general bases in either of these enzymes (Glu 166 and Tyr 150, respectively). Our structures of the native and chloride inhibited forms of OXA-10 suggest that the class D enzymes have evolved a distinct catalytic mechanism for beta-lactam hydrolysis. Clinical variants of OXA-10 are also discussed in light of the structure.

Crystal structure of the class D beta-lactamase OXA-10.,Paetzel M, Danel F, de Castro L, Mosimann SC, Page MG, Strynadka NC Nat Struct Biol. 2000 Oct;7(10):918-25. PMID:11017203^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Paetzel M, Danel F, de Castro L, Mosimann SC, Page MG, Strynadka NC. Crystal structure of the class D beta-lactamase OXA-10. Nat Struct Biol. 2000 Oct;7(10):918-25. PMID:11017203 doi:10.1038/79688

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Paetzel M, Danel F, de Castro L, Mosimann SC, Page MG, Strynadka NC. Crystal structure of the class D beta-lactamase OXA-10. Nat Struct Biol. 2000 Oct;7(10):918-25. PMID:11017203 doi:10.1038/79688

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='1fof' size='340' side='right'caption='[[1fof]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1fof]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FOF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FOF FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1fof]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FOF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FOF FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fof OCA], [http://pdbe.org/1fof PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fof RCSB], [http://www.ebi.ac.uk/pdbsum/1fof PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fof ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fof OCA], [https://pdbe.org/1fof PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fof RCSB], [https://www.ebi.ac.uk/pdbsum/1fof PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fof ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/BLO10_PSEAI BLO10_PSEAI]] Hydrolyzes both carbenicillin and oxacillin.
+[https://www.uniprot.org/uniprot/BLO10_PSEAI BLO10_PSEAI] Hydrolyzes both carbenicillin and oxacillin.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 15: / Line 15: @@
    <jmolCheckbox>
      <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fo/1fof_consurf.spt"</scriptWhenChecked>
-     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
      <text>to colour the structure by Evolutionary Conservation</text>
    </jmolCheckbox>
@@ Line 36: / Line 36: @@
 __TOC__
 </StructureSection>
-[[Category: Beta-lactamase]]
 [[Category: Large Structures]]
-[[Category: Castro, L de]]
+[[Category: Pseudomonas aeruginosa]]
-[[Category: Danel, F]]
+[[Category: Danel F]]
-[[Category: Mosimann, S C]]
+[[Category: Mosimann SC]]
-[[Category: Paetzel, M]]
+[[Category: Paetzel M]]
-[[Category: Page, M G.P]]
+[[Category: Page MGP]]
-[[Category: Strynadka, N C.J]]
+[[Category: Strynadka NCJ]]
-[[Category: Class-d]]
+[[Category: De Castro L]]
-[[Category: Cobalt]]
-[[Category: Hydrolase]]
-[[Category: Oxa-10]]
-[[Category: Oxacillinase]]

1fof: Difference between revisions

Latest revision as of 07:16, 9 October 2024

CRYSTAL STRUCTURE OF THE CLASS D BETA-LACTAMASE OXA-10

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

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