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==SOLUTION STRUCTURE OF HUMAN LYMPHOTACTIN==
==SOLUTION STRUCTURE OF HUMAN LYMPHOTACTIN==
<StructureSection load='1j9o' size='340' side='right'caption='[[1j9o]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
<StructureSection load='1j9o' size='340' side='right'caption='[[1j9o]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1j9o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J9O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J9O FirstGlance]. <br>
<table><tr><td colspan='2'>[[1j9o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J9O FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1j8i|1j8i]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j9o OCA], [http://pdbe.org/1j9o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1j9o RCSB], [http://www.ebi.ac.uk/pdbsum/1j9o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1j9o ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j9o OCA], [https://pdbe.org/1j9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j9o RCSB], [https://www.ebi.ac.uk/pdbsum/1j9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j9o ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/XCL1_HUMAN XCL1_HUMAN]] Chemotactic activity for lymphocytes but not for monocytes or neutrophils.  
[https://www.uniprot.org/uniprot/XCL1_HUMAN XCL1_HUMAN] Chemotactic activity for lymphocytes but not for monocytes or neutrophils.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j9o ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j9o ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lymphotactin, the sole identified member of the C class of chemokines, specifically attracts T lymphocytes and natural killer cells. This 93-residue protein lacks 2 of the 4 conserved cysteine residues characteristic of the other 3 classes of chemokines and possesses an extended carboxyl terminus, which is required for chemotactic activity. We have determined the three-dimensional solution structure of recombinant human lymphotactin by NMR spectroscopy. Under the conditions used for the structure determination, lymphotactin was predominantly monomeric; however, pulsed field gradient NMR self-diffusion measurements and analytical ultracentrifugation revealed evidence of dimer formation. Sequence-specific chemical shift assignments were determined through analysis of two- and three-dimensional NMR spectra of (15)N- and (13)C/(15)N-enriched protein samples. Input for the torsion angle dynamics calculations used in determining the structure included 1258 unique NOE-derived distance constraints and 60 dihedral angle constraints obtained from chemical-shift-based searching of a protein conformational database. The ensemble of 20 structures chosen to represent the structure had backbone and heavy atom rms deviations of 0.46 +/- 0.11 and 1.02 +/- 0.14 A, respectively. The results revealed that human lymphotactin adopts the conserved chemokine fold, which is characterized by a three-stranded antiparallel beta-sheet and a C-terminal alpha-helix. Two regions are dynamically disordered as evidenced by (1)H and (13)C chemical shifts and [(15)N]-(1)H NOEs: residues 1-9 of the amino terminus and residues 69-93 of the C-terminal extension. A functional role for the C-terminal extension, which is unique to lymphotactin, remains to be elucidated.
Monomeric solution structure of the prototypical 'C' chemokine lymphotactin.,Kuloglu ES, McCaslin DR, Kitabwalla M, Pauza CD, Markley JL, Volkman BF Biochemistry. 2001 Oct 23;40(42):12486-96. PMID:11601972<ref>PMID:11601972</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1j9o" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Human]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Kitabwalla, M]]
[[Category: Kitabwalla M]]
[[Category: Kuloglu, E S]]
[[Category: Kuloglu ES]]
[[Category: Markley, J L]]
[[Category: Markley JL]]
[[Category: McCaslin, D R]]
[[Category: McCaslin DR]]
[[Category: Pauza, C D]]
[[Category: Pauza CD]]
[[Category: Volkman, B F]]
[[Category: Volkman BF]]
[[Category: Chemokine]]
[[Category: Cytokine]]
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