3t15: Difference between revisions

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<StructureSection load='3t15' size='340' side='right'caption='[[3t15]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
<StructureSection load='3t15' size='340' side='right'caption='[[3t15]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3t15]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/American_tobacco American tobacco]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T15 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3t15]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T15 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zw6|3zw6]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rca1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 American tobacco])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t15 OCA], [https://pdbe.org/3t15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t15 RCSB], [https://www.ebi.ac.uk/pdbsum/3t15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t15 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t15 OCA], [http://pdbe.org/3t15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t15 RCSB], [http://www.ebi.ac.uk/pdbsum/3t15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t15 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/RCA1_TOBAC RCA1_TOBAC]] Activation of RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase; EC 4.1.1.39) involves the ATP-dependent carboxylation of the epsilon-amino group of lysine leading to a carbamate structure.  
[https://www.uniprot.org/uniprot/RCA1_TOBAC RCA1_TOBAC] Activation of RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase; EC 4.1.1.39) involves the ATP-dependent carboxylation of the epsilon-amino group of lysine leading to a carbamate structure.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Rubisco, the enzyme that catalyzes the fixation of atmospheric CO(2) in photosynthesis, is subject to inactivation by inhibitory sugar phosphates. Here we report the 2.95-A crystal structure of Nicotiana tabacum Rubisco activase (Rca), the enzyme that facilitates the removal of these inhibitors. Rca from tobacco has a classical AAA(+)-protein domain architecture. Although Rca populates a range of oligomeric states when in solution, it forms a helical arrangement with six subunits per turn when in the crystal. However, negative-stain electron microscopy of the active mutant R294V suggests that Rca functions as a hexamer. The residues determining species specificity for Rubisco are located in a helical insertion of the C-terminal domain and probably function in conjunction with the N-domain in Rubisco recognition. Loop segments exposed toward the central pore of the hexamer are required for the ATP-dependent remodeling of Rubisco, resulting in the release of inhibitory sugar.
 
Structure of green-type Rubisco activase from tobacco.,Stotz M, Mueller-Cajar O, Ciniawsky S, Wendler P, Hartl FU, Bracher A, Hayer-Hartl M Nat Struct Mol Biol. 2011 Nov 6. doi: 10.1038/nsmb.2171. PMID:22056769<ref>PMID:22056769</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3t15" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[RuBisCO|RuBisCO]]
*[[RuBisCO 3D structures|RuBisCO 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: American tobacco]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Bracher, A]]
[[Category: Nicotiana tabacum]]
[[Category: Hartl, F U]]
[[Category: Bracher A]]
[[Category: Hayer-Hartl, M]]
[[Category: Hartl FU]]
[[Category: Mueller-Cajar, O]]
[[Category: Hayer-Hartl M]]
[[Category: Stotz, M]]
[[Category: Mueller-Cajar O]]
[[Category: Wendler, P]]
[[Category: Stotz M]]
[[Category: Aaa+ protein]]
[[Category: Wendler P]]
[[Category: Photosynthesis]]
[[Category: Rubisco activase]]
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