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| <StructureSection load='4my5' size='340' side='right'caption='[[4my5]], [[Resolution|resolution]] 2.19Å' scene=''> | | <StructureSection load='4my5' size='340' side='right'caption='[[4my5]], [[Resolution|resolution]] 2.19Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4my5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Strmu Strmu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MY5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MY5 FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4my5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_mutans_UA159 Streptococcus mutans UA159]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MY5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MY5 FirstGlance]. <br> |
| </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SMU_24 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=210007 STRMU])</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.194Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4my5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4my5 OCA], [http://pdbe.org/4my5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4my5 RCSB], [http://www.ebi.ac.uk/pdbsum/4my5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4my5 ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4my5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4my5 OCA], [https://pdbe.org/4my5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4my5 RCSB], [https://www.ebi.ac.uk/pdbsum/4my5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4my5 ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/Q8DWM1_STRMU Q8DWM1_STRMU] |
| Streptococcus mutans, a facultatively aerobic and Gram-positive bacterium, is the primary causative agent of dental caries and contributes to the multispecies biofilm known as dental plaque. In this study, the aromatic-amino-acid aminotransferase from Streptococcus mutans (SmAroAT) was recombinantly expressed in Escherichia coli. An effective purification protocol was established. The recombinant protein was crystallized using the hanging-drop vapor-diffusion method with PEG 3350 as the primary precipitant. The crystal structure of SmAroAT was solved at 2.2 A resolution by the molecular-replacement method. Structural analysis indicated that the proteins of the aromatic-amino-acid aminotransferase family have conserved structural elements that might play a role in substrate binding. These results may help in obtaining a better understanding of the catabolism and biosynthesis of aromatic amino acids.
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| Crystal structure of the aromatic-amino-acid aminotransferase from Streptococcus mutans.,Cong X, Li X, Li S Acta Crystallogr F Struct Biol Commun. 2019 Feb 1;75(Pt 2):141-146. doi:, 10.1107/S2053230X18018472. Epub 2019 Jan 24. PMID:30713166<ref>PMID:30713166</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 4my5" style="background-color:#fffaf0;"></div>
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| == References ==
| |
| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Strmu]] | | [[Category: Streptococcus mutans UA159]] |
| [[Category: Cong, X]] | | [[Category: Cong X]] |
| [[Category: Feng, X]] | | [[Category: Feng X]] |
| [[Category: Feng, Y]] | | [[Category: Feng Y]] |
| [[Category: Ge, J]] | | [[Category: Ge J]] |
| [[Category: Li, S]] | | [[Category: Li S]] |
| [[Category: Li, X]] | | [[Category: Li X]] |
| [[Category: Amino acid metabolism]]
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| [[Category: Oral infectious disease]]
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| [[Category: Smaroat]]
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| [[Category: Transferase]]
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| [[Category: Type 1 plp-dependent aminotransferase]]
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