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==Nucleotide-free Kinesin motor domain complexed with GMPCPP-microtubule==
==Nucleotide-free Kinesin motor domain complexed with GMPCPP-microtubule==
<StructureSection load='3j6h' size='340' side='right'caption='[[3j6h]], [[Resolution|resolution]] 8.10&Aring;' scene=''>
<SX load='3j6h' size='340' side='right' viewer='molstar' caption='[[3j6h]], [[Resolution|resolution]] 8.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3j6h]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J6H FirstGlance]. <br>
<table><tr><td colspan='2'>[[3j6h]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J6H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J6H FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.1&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Kif5c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6h OCA], [http://pdbe.org/3j6h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j6h RCSB], [http://www.ebi.ac.uk/pdbsum/3j6h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6h ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j6h OCA], [https://pdbe.org/3j6h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j6h RCSB], [https://www.ebi.ac.uk/pdbsum/3j6h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j6h ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/TBA1A_PIG TBA1A_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/KIF5C_MOUSE KIF5C_MOUSE]] Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Mediates dendritic trafficking of mRNAs.<ref>PMID:19608740</ref>  [[http://www.uniprot.org/uniprot/TBB_PIG TBB_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
[https://www.uniprot.org/uniprot/TBA1A_PIG TBA1A_PIG] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The molecular motor kinesin moves along microtubules using energy from ATP hydrolysis in an initial step coupled with ADP release. In neurons, kinesin-1/KIF5C preferentially binds to the GTP-state microtubules over GDP-state microtubules to selectively enter an axon among many processes; however, because the atomic structure of nucleotide-free KIF5C is unavailable, its molecular mechanism remains unresolved. Here, the crystal structure of nucleotide-free KIF5C and the cryo-electron microscopic structure of nucleotide-free KIF5C complexed with the GTP-state microtubule are presented. The structures illustrate mutual conformational changes induced by interaction between the GTP-state microtubule and KIF5C. KIF5C acquires the 'rigor conformation', where mobile switches I and II are stabilized through L11 and the initial portion of the neck-linker, facilitating effective ADP release and the weak-to-strong transition of KIF5C microtubule affinity. Conformational changes to tubulin strengthen the longitudinal contacts of the GTP-state microtubule in a similar manner to GDP-taxol microtubules. These results and functional analyses provide the molecular mechanism of the preferential binding of KIF5C to GTP-state microtubules.
 
X-ray and Cryo-EM structures reveal mutual conformational changes of Kinesin and GTP-state microtubules upon binding.,Morikawa M, Yajima H, Nitta R, Inoue S, Ogura T, Sato C, Hirokawa N EMBO J. 2015 Mar 16. pii: e201490588. PMID:25777528<ref>PMID:25777528</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3j6h" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Kinesin 3D Structures|Kinesin 3D Structures]]
*[[Kinesin 3D Structures|Kinesin 3D Structures]]
*[[Tubulin|Tubulin]]
*[[Tubulin 3D Structures|Tubulin 3D Structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Lk3 transgenic mice]]
[[Category: Mus musculus]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
[[Category: Hirokawa, N]]
[[Category: Hirokawa N]]
[[Category: Inoue, S]]
[[Category: Inoue S]]
[[Category: Morikawa, M]]
[[Category: Morikawa M]]
[[Category: Nitta, R]]
[[Category: Nitta R]]
[[Category: Ogura, T]]
[[Category: Ogura T]]
[[Category: Sato, C]]
[[Category: Sato C]]
[[Category: Yajima, H]]
[[Category: Yajima H]]
[[Category: Axonal transport]]
[[Category: Gmpcpp-microtubule]]
[[Category: Kinesin]]
[[Category: Microtubule]]
[[Category: Motor domain]]
[[Category: Nucleotide-free kinesin]]
[[Category: Rigor-conformation]]
[[Category: Structural protein-motor protein complex]]
[[Category: Tubulin]]

Latest revision as of 08:28, 20 March 2024

Nucleotide-free Kinesin motor domain complexed with GMPCPP-microtubule

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