5d99: Difference between revisions

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<StructureSection load='5d99' size='340' side='right'caption='[[5d99]], [[Resolution|resolution]] 0.97&Aring;' scene=''>
<StructureSection load='5d99' size='340' side='right'caption='[[5d99]], [[Resolution|resolution]] 0.97&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5d99]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D99 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D99 FirstGlance]. <br>
<table><tr><td colspan='2'>[[5d99]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_L1 Enterobacteria phage L1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D99 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.97&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3dw4|3dw4]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d99 OCA], [https://pdbe.org/5d99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d99 RCSB], [https://www.ebi.ac.uk/pdbsum/5d99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d99 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d99 OCA], [http://pdbe.org/5d99 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d99 RCSB], [http://www.ebi.ac.uk/pdbsum/5d99 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5d99 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Site-specific 2'-methylseleno RNA labeling is a promising tool for tackling the phase problem in RNA crystallography. We have developed an efficient strategy for crystallization and structure determination of RNA and RNA/protein complexes based on preliminary crystallization screening of 2'-OCH(3)-modified RNA sequences, prior to the replacement of 2'-OCH(3) groups with their 2'-SeCH(3) counterparts. The method exploits the similar crystallization properties of 2'-OCH(3)- and 2'-SeCH(3)-modified RNAs and has been successfully validated for two test cases. In addition, our data show that 2'-SeCH(3)-modified RNA have an increased resistance to X-ray radiolysis in comparison with commonly used 5-halogen-modified RNA, which permits collection of experimental electron density maps of remarkable quality.
A fast selenium derivatization strategy for crystallization and phasing of RNA structures.,Olieric V, Rieder U, Lang K, Serganov A, Schulze-Briese C, Micura R, Dumas P, Ennifar E RNA. 2009 Apr;15(4):707-15. Epub 2009 Feb 18. PMID:19228585<ref>PMID:19228585</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5d99" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Enterobacteria phage L1]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Mooers, B H.M]]
[[Category: Mooers BHM]]
[[Category: Ab initio direct methods structure determination]]
[[Category: Hairpin rna]]
[[Category: Rna]]
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