6tr1: Difference between revisions

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New page: ==Native cytochrome c6 from Thermosynechococcus elongatus in space group H3== <StructureSection load='6tr1' size='340' side='right'caption='6tr1, resolution 1.70Å...
 
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<StructureSection load='6tr1' size='340' side='right'caption='[[6tr1]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
<StructureSection load='6tr1' size='340' side='right'caption='[[6tr1]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6tr1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus_(strain_bp-1) Thermosynechococcus elongatus (strain bp-1)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TR1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TR1 FirstGlance]. <br>
<table><tr><td colspan='2'>[[6tr1]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TR1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TR1 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MEN:N-METHYL+ASPARAGINE'>MEN</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=MEN:N-METHYL+ASPARAGINE'>MEN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6tr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tr1 OCA], [http://pdbe.org/6tr1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tr1 RCSB], [http://www.ebi.ac.uk/pdbsum/6tr1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tr1 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tr1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tr1 OCA], [https://pdbe.org/6tr1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tr1 RCSB], [https://www.ebi.ac.uk/pdbsum/6tr1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tr1 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/CYC6_THEEB CYC6_THEEB]] Functions as an electron carrier between membrane-bound cytochrome b6-f and photosystem I in oxygenic photosynthesis.  
[https://www.uniprot.org/uniprot/CYC6_THEVB CYC6_THEVB] Functions as an electron carrier between membrane-bound cytochrome b6-f and photosystem I in oxygenic photosynthesis.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Native cytochrome c6 was purified from an extract of strain BP-1 of the thermophilic cyanobacterium Thermosynechococcus elongatus. The protein was crystallized, and with only slight modifications of the buffer and vapour-diffusion conditions two different space groups were observed, namely H3 and C2. Both crystal structures were solved; they contained three and six molecules per asymmetric unit and were refined to 1.7 and 2.25 A resolution, respectively. To date, the structure of native cytochrome c6 from T. elongatus has only been reported as a monomer using NMR spectroscopy, i.e. without addressing putative oligomerization, and related structures have only previously been solved using X-ray crystallography after recombinant gene overexpression in Escherichia coli. The reported space groups of related cyanobacterial cytochrome c6 structures differ from those reported here. Interestingly, the protein-protein interfaces that were observed utilizing X-ray crystallography could also explain homo-oligomerization in solution; specifically, trimerization is indicated by infra-red dynamic light scattering and blue native gel electrophoresis in solution. Trimers were also detected by mass spectrometry. Furthermore, there is an indication of post-translational methylation in the crystal structure. Additionally, the possibility of modifying the crystal size and the redox activity in the context of photosynthesis is shaping the investigated cytochrome as a highly suitable model protein for advanced serial crystallography at highly brilliant X-ray free-electron laser sources.
 
Crystal structures of native cytochrome c6 from Thermosynechococcus elongatus in two different space groups and implications for its oligomerization.,Falke S, Feiler C, Chapman H, Sarrou I Acta Crystallogr F Struct Biol Commun. 2020 Sep 1;76(Pt 9):444-452. doi:, 10.1107/S2053230X20010249. Epub 2020 Aug 20. PMID:32880593<ref>PMID:32880593</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6tr1" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Cytochrome f 3D structures|Cytochrome f 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Falke, S]]
[[Category: Thermosynechococcus vestitus BP-1]]
[[Category: Feiler, C G]]
[[Category: Falke S]]
[[Category: Sarrou, I]]
[[Category: Feiler CG]]
[[Category: Cyanobacteria]]
[[Category: Sarrou I]]
[[Category: Electron transport]]
[[Category: Heme]]
[[Category: Photosynthesis]]
[[Category: Photosystem i]]
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