1a7u: Difference between revisions

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[[Image:1a7u.jpg|left|200px]]
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{{STRUCTURE_1a7u|  PDB=1a7u  |  SCENE=  }}
'''CHLOROPEROXIDASE T'''


==CHLOROPEROXIDASE T==
<StructureSection load='1a7u' size='340' side='right'caption='[[1a7u]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1a7u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kitasatospora_aureofaciens Kitasatospora aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A7U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A7U FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a7u OCA], [https://pdbe.org/1a7u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a7u RCSB], [https://www.ebi.ac.uk/pdbsum/1a7u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a7u ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PRXC_KITAU PRXC_KITAU]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a7/1a7u_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a7u ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structures of cofactor-free haloperoxidases from Streptomyces aureofaciens, Streptomyces lividans, and Pseudomonas fluorescens have been determined at resolutions between 1.9 A and 1.5 A. The structures of two enzymes complexed with benzoate or propionate identify the binding site for the organic acids which are required for the haloperoxidase activity. Based on these complexes and on the structure of an inactive variant, a reaction mechanism is proposed for the halogenation reaction with peroxoacid and hypohalous acid as reaction intermediates. Comparison of the structures suggests that a specific halide binding site is absent in the enzymes but that hydrophobic organic compounds may fit into the active site pocket for halogenation at preferential sites.


==Overview==
Structural investigation of the cofactor-free chloroperoxidases.,Hofmann B, Tolzer S, Pelletier I, Altenbuchner J, van Pee KH, Hecht HJ J Mol Biol. 1998 Jun 19;279(4):889-900. PMID:9642069<ref>PMID:9642069</ref>
The structures of cofactor-free haloperoxidases from Streptomyces aureofaciens, Streptomyces lividans, and Pseudomonas fluorescens have been determined at resolutions between 1.9 A and 1.5 A. The structures of two enzymes complexed with benzoate or propionate identify the binding site for the organic acids which are required for the haloperoxidase activity. Based on these complexes and on the structure of an inactive variant, a reaction mechanism is proposed for the halogenation reaction with peroxoacid and hypohalous acid as reaction intermediates. Comparison of the structures suggests that a specific halide binding site is absent in the enzymes but that hydrophobic organic compounds may fit into the active site pocket for halogenation at preferential sites.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1A7U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A7U OCA].
</div>
<div class="pdbe-citations 1a7u" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structural investigation of the cofactor-free chloroperoxidases., Hofmann B, Tolzer S, Pelletier I, Altenbuchner J, van Pee KH, Hecht HJ, J Mol Biol. 1998 Jun 19;279(4):889-900. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9642069 9642069]
*[[Haloperoxidase|Haloperoxidase]]
[[Category: Chloride peroxidase]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Streptomyces aureofaciens]]
__TOC__
[[Category: Altenbuchner, J.]]
</StructureSection>
[[Category: Hecht, H J.]]
[[Category: Kitasatospora aureofaciens]]
[[Category: Hofmann, B.]]
[[Category: Large Structures]]
[[Category: Pee, K H.Van.]]
[[Category: Altenbuchner J]]
[[Category: Pelletier, I.]]
[[Category: Hecht H-J]]
[[Category: Toelzer, S.]]
[[Category: Hofmann B]]
[[Category: Alpha/beta hydrolase fold]]
[[Category: Pelletier I]]
[[Category: Haloperoxidase]]
[[Category: Toelzer S]]
[[Category: Oxidoreductase]]
[[Category: Van Pee K-H]]
[[Category: Peroxidase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 09:56:40 2008''
Retrieved from "https://proteopedia.org/1a7u"