Thiolase: Difference between revisions

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== Function ==

Two different classes of '''thiolases''' are found.

*'''Degrative thiolase''' like '''3-ketoacyl-CoA thiolase''' (KCT) is involved in fatty acid β oxidation<ref>PMID:16630629</ref>.

*'''Degrative thiolase''' like '''3-ketoacyl-CoA thiolase''' (KCT) is involved in fatty acid [[beta oxidation]]<ref>PMID:16630629</ref>.

*'''Biosynthetic thiolases''' like '''acetoacetyl-CoA thiolase''' (ACT) are involved in the thiolysis of acetoacetyl-CoA<ref>PMID:21908473</ref>.

*'''Biosynthetic thiolases''' like '''acetoacetyl-CoA thiolase''' (ACT) or '''acetyl-CoA acetyltransferase''' are involved in the thiolysis of acetoacetyl-CoA<ref>PMID:21908473</ref>.

For bacterial thiolase see [[Bacterial thiolase]]

For zebrafish thiolase see [[SCP2-thiolase]]

*'''SCP2-thiolase''' converts in the bile acid synthesis pathway 24-keto-THC-CoA into choloyl-CoA and propionyl-CoA<ref>PMID:30573650</ref>. For zebrafish thiolase see [[SCP2-thiolase]]. ''' SCP''' is Sterol Carrier Protein.

*'''U34-tRNA-thiolase''' thiolates uridine 34 in tRNA anticodon loop which guarantees the fidelity of protein translation in archaea<ref>PMID:37005440</ref>.

See also [[Mevalonate pathway]]

== Structural highlights ==

The <scene name='44/447919/Cv/8'>active site of 3-ketoacyl-CoA thiolase contains CoA</scene><ref>PMID:25478839</ref>. Water molecules are shown as red spheres.

~~</StructureSection>~~

== 3D Structures of Thiolase ==

[[Thiolase 3D structures]]

~~Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}~~

</StructureSection>

~~{{#tree:id=OrganizedByTopic|openlevels=0|~~

*3-ketoacyl-CoA thiolase

**[[2wu9]], [[2c7y]], [[2c7z]] – KCT peroxisomal residues 36-462 – ''Arabidopsis thaliana''

**[[2wua]] - KCT peroxisomal residues 17-449 (mutant) – sunflower

**[[2iik]] – hKCT peroxisomal – human

**[[4c2k]] – hKCT mitochondrial

**[[4c2j]] – hKCT mitochondrial + CoA

**[[3goa]] – KCT – ''Salmonella typhimurium''

**[[5lp7]] – KCT – ''Bacillus subtilis''

**[[1afw]], [[1pxt]] – yKCT peroxisomal – yeast

**[[5cbq]] – MsKCT – ''Mycobacterium smegmatis''

**[[4w61]] – ReKCT – ''Ralstonia eutropha''

**[[4ubw]], [[5onc]] – MtKCT – ''Mycobacterium tuberculosis''

*3-ketoacyl-CoA thiolase complex

**[[3w15]] - yKCT peroxisomal residues 27-396 + peroxiomal membrane protein PEX21 + peroxisomal targeting signal receptor

**[[2d3t]] – PfKCT + fatty oxidation complex β subunit – ''Pseudomonas fragi''

**[[1wdk]], [[1wdl]], [[1wdm]] - PfKCT + fatty oxidation complex α subunit

**[[5bz4]] – MsKCT + CoA

**[[4ubt]] – MtKCT (mutant) + CoA + steroid

**[[4ubu]] – MtKCT (mutant) + CoA

**[[4ubv]] – MtKCT + CoA + acetyl CoA

*Acetoacetyl-CoA thiolase or acetyl-CoA acetyltransferase

**[[1m4s]], [[1m4t]], [[1dlu]], [[1qfl]] - ZrACT – ''Zoogloea ramigera''

**[[2wku]], [[2wl6]], [[1m1t]], [[1m3k]] - ZrACT (mutant)

**[[2ib7]], [[2ib8]], [[2ib9]], [[2ibu]], [[2ibw]], [[2iby]], [[2f2s]] – hACT mitochondrial

**[[1wl5]] - hACT cytosolic

**[[4dd5]], [[4e1l]] – ACT – ''Clostridium difficile''

**[[4xl2]], [[4xl3]], [[4n44]], [[4n45]] – CaACT – ''Clostridium acetobutylicum''

**[[4wyr]] – CaACT (mutant)

**[[5f0v]], [[5f38]], [[4wys]] – ACT – ''Escherichia coli''

**[[4o9a]], [[4o99]], [[4o9c]], [[4nzs]] – ReACT

**[[5xyj]], [[5xz5]] – yACT

*Acetoacetyl-CoA thiolase complex

**[[1dm3]], [[1dlv]] – ZrACT + CoA

**[[2wkt]], [[2wkv]], [[2wl4]], [[2wl5]], [[2vtz]], [[1m3z]] – ZrACT (mutant) + CoA

**[[1m1o]] - ZrACT (mutant) + acetoacetyl-CoA

**[[2vu0]], [[1nl7]] - ZrACT + CoA

**[[2vu1]], [[2vu2]], [[1ou6]] - ZrACT + pantheteine-11-pivalate

**[[1wl4]] – hACT cytosolic + CoA

**[[4xl4]] – CaACT + CoA

**[[6et9]] – MtACT + PFAM DUF35 + hydroxymethylglutaryl-CoA synthase - ''Methanothermococcus thermolithotrophicus''

**[[6esq]] – MtACT + CoA + PFAM DUF35 + hydroxymethylglutaryl-CoA synthase

**[[6are]] – ACT + CoA + acetate - ''Aspergillus fumigatus''

}}

== References ==

[[Category:Topic Page]]

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-<StructureSection load='4c2j' size='350' side='right' caption='Human 3-ketoacyl-CoA complex with CoA and ethylene glycol(PDB code [[4c2j]]).' scene='44/447919/Cv/7'>
+<StructureSection load='4c2j' size='350' side='right' caption='Human 3-ketoacyl-CoA complex with CoA and ethylene glycol (PDB code [[4c2j]])' scene='44/447919/Cv/7'>
 == Function ==
 Two different classes of '''thiolases''' are found.<br />
-*'''Degrative thiolase''' like '''3-ketoacyl-CoA thiolase''' (KCT) is involved in fatty acid β oxidation<ref>PMID:16630629</ref>.<br />
+*'''Degrative thiolase''' like '''3-ketoacyl-CoA thiolase''' (KCT) is involved in fatty acid [[beta oxidation]]<ref>PMID:16630629</ref>.<br />
-*'''Biosynthetic thiolases''' like '''acetoacetyl-CoA thiolase''' (ACT) are involved in the thiolysis of acetoacetyl-CoA<ref>PMID:21908473</ref>.
+*'''Biosynthetic thiolases''' like '''acetoacetyl-CoA thiolase''' (ACT) or '''acetyl-CoA acetyltransferase''' are involved in the thiolysis of acetoacetyl-CoA<ref>PMID:21908473</ref>.
 For bacterial thiolase see [[Bacterial thiolase]]
-For zebrafish thiolase see [[SCP2-thiolase]]
+*'''SCP2-thiolase''' converts in the bile acid synthesis pathway 24-keto-THC-CoA into choloyl-CoA and propionyl-CoA<ref>PMID:30573650</ref>.  For zebrafish thiolase see [[SCP2-thiolase]]. ''' SCP''' is Sterol Carrier Protein.
+*'''U34-tRNA-thiolase''' thiolates uridine 34 in tRNA anticodon loop which guarantees the fidelity of protein translation in archaea<ref>PMID:37005440</ref>.
+See also [[Mevalonate pathway]]
 == Structural highlights ==
 The <scene name='44/447919/Cv/8'>active site of 3-ketoacyl-CoA thiolase contains CoA</scene><ref>PMID:25478839</ref>. Water molecules are shown as red spheres.
-</StructureSection>
 == 3D Structures of Thiolase ==
+[[Thiolase 3D structures]]
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+</StructureSection>
-{{#tree:id=OrganizedByTopic|openlevels=0|
-*3-ketoacyl-CoA thiolase
-**[[2wu9]], [[2c7y]], [[2c7z]] – KCT peroxisomal residues 36-462 – ''Arabidopsis thaliana''<br />
-**[[2wua]] - KCT peroxisomal residues 17-449 (mutant) – sunflower<br />
-**[[2iik]] – hKCT peroxisomal – human<br />
-**[[4c2k]] – hKCT mitochondrial <br />
-**[[4c2j]] – hKCT mitochondrial + CoA<br />
-**[[3goa]] – KCT – ''Salmonella typhimurium''<br />
-**[[5lp7]] – KCT – ''Bacillus subtilis'' <br />
-**[[1afw]], [[1pxt]] – yKCT peroxisomal – yeast<br />
-**[[5cbq]] – MsKCT – ''Mycobacterium smegmatis'' <br />
-**[[4w61]] – ReKCT – ''Ralstonia eutropha''<br />
-**[[4ubw]], [[5onc]] – MtKCT – ''Mycobacterium tuberculosis'' <br />
-*3-ketoacyl-CoA thiolase complex
-**[[3w15]] - yKCT peroxisomal residues 27-396 + peroxiomal membrane protein PEX21 + peroxisomal targeting signal receptor<br />
-**[[2d3t]] – PfKCT + fatty oxidation complex β subunit – ''Pseudomonas fragi''<br />
-**[[1wdk]], [[1wdl]], [[1wdm]] - PfKCT + fatty oxidation complex α subunit<br />
-**[[5bz4]] – MsKCT + CoA  <br />
-**[[4ubt]] – MtKCT (mutant) + CoA + steroid  <br />
-**[[4ubu]] – MtKCT (mutant) + CoA  <br />
-**[[4ubv]] – MtKCT + CoA + acetyl CoA  <br />
-*Acetoacetyl-CoA thiolase or acetyl-CoA acetyltransferase
-**[[1m4s]], [[1m4t]], [[1dlu]], [[1qfl]] - ZrACT – ''Zoogloea ramigera''<br />
-**[[2wku]], [[2wl6]], [[1m1t]], [[1m3k]] - ZrACT (mutant) <br />
-**[[2ib7]], [[2ib8]], [[2ib9]], [[2ibu]], [[2ibw]], [[2iby]], [[2f2s]] – hACT mitochondrial<br />
-**[[1wl5]] - hACT cytosolic<br />
-**[[4dd5]], [[4e1l]] – ACT – ''Clostridium difficile''<br />
-**[[4xl2]], [[4xl3]], [[4n44]], [[4n45]] – CaACT – ''Clostridium acetobutylicum''<br />
-**[[4wyr]] – CaACT (mutant) <br />
-**[[5f0v]], [[5f38]], [[4wys]] – ACT – ''Escherichia coli'' <br />
-**[[4o9a]], [[4o99]], [[4o9c]], [[4nzs]] – ReACT <br />
-**[[5xyj]], [[5xz5]] – yACT <br />
-*Acetoacetyl-CoA thiolase complex
-**[[1dm3]], [[1dlv]] – ZrACT + CoA<br />
-**[[2wkt]], [[2wkv]], [[2wl4]], [[2wl5]], [[2vtz]], [[1m3z]] – ZrACT (mutant) + CoA<br />
-**[[1m1o]] - ZrACT (mutant) + acetoacetyl-CoA<br />
-**[[2vu0]], [[1nl7]] - ZrACT + CoA<br />
-**[[2vu1]], [[2vu2]], [[1ou6]] - ZrACT + pantheteine-11-pivalate<br />
-**[[1wl4]] – hACT cytosolic + CoA <br />
-**[[4xl4]] – CaACT + CoA <br />
-**[[6et9]] – MtACT + PFAM DUF35 + hydroxymethylglutaryl-CoA synthase - ''Methanothermococcus thermolithotrophicus''<br />
-**[[6esq]] – MtACT + CoA + PFAM DUF35 + hydroxymethylglutaryl-CoA synthase <br />
-**[[6are]] – ACT + CoA + acetate - ''Aspergillus fumigatus'' <br />
-}}
 == References ==
 <references/>
 [[Category:Topic Page]]