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New page: left|200px<br /> <applet load="1vj4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1vj4, resolution 1.800Å" /> '''SEQUENCE-DEPENDENT...
 
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[[Image:1vj4.gif|left|200px]]<br />
<applet load="1vj4" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1vj4, resolution 1.800&Aring;" />
'''SEQUENCE-DEPENDENT CONFORMATION OF AN A-DNA DOUBLE HELIX: THE CRYSTAL STRUCTURE OF THE OCTAMER D(G-G-T-A-T-A-C-C)'''<br />


==Overview==
==SEQUENCE-DEPENDENT CONFORMATION OF AN A-DNA DOUBLE HELIX: THE CRYSTAL STRUCTURE OF THE OCTAMER D(G-G-T-A-T-A-C-C)==
The crystal structures of the synthetic self-complementary octamer, d(G-G-T-A-T-A-C-C) and its 5-bromouracil-containing analogue have been, refined to R values of 20% and 14% at resolutions of 1.8 and 2.25 A, respectively. The molecules adopt and A-DNA type double-helical, conformation, which is minimally affected by crystal forces. A detailed, analysis of the structure shows a considerable influence of the nucleotide, sequence on the base-pair stacking patterns. In particular, the, electrostatic stacking interactions between adjacent guanine and thymine, bases produce symmetric bending of the double helix and a major-groove, widening. The sugar-phosphate backbone appears to be only slightly, affected by the base sequence. The local variations in the base-pair, orientation are brought about by correlated adjustments in the backbone, torsion angles and the glycosidic orientation. Sequence-dependent, conformational variations of the type observed here may contribute to the, specificity of certain protein-DNA interactions.
<StructureSection load='1vj4' size='340' side='right'caption='[[1vj4]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1vj4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VJ4 FirstGlance]. <br>
1VJ4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1VJ4 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vj4 OCA], [https://pdbe.org/1vj4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vj4 RCSB], [https://www.ebi.ac.uk/pdbsum/1vj4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vj4 ProSAT]</span></td></tr>
==Reference==
</table>
Sequence-dependent conformation of an A-DNA double helix. The crystal structure of the octamer d(G-G-T-A-T-A-C-C)., Shakked Z, Rabinovich D, Kennard O, Cruse WB, Salisbury SA, Viswamitra MA, J Mol Biol. 1983 May 15;166(2):183-201. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=6854642 6854642]
__TOC__
[[Category: Protein complex]]
</StructureSection>
[[Category: Cruse, W.B.]]
[[Category: Large Structures]]
[[Category: Kennard, O.]]
[[Category: Cruse WB]]
[[Category: Rabinovich, D.]]
[[Category: Kennard O]]
[[Category: Salisbury, S.A.]]
[[Category: Rabinovich D]]
[[Category: Shakked, Z.]]
[[Category: Salisbury SA]]
[[Category: Viswamitra, M.A.]]
[[Category: Shakked Z]]
[[Category: a-dna]]
[[Category: Viswamitra MA]]
[[Category: double helix]]
 
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