6l00: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 3: | Line 3: | ||
<StructureSection load='6l00' size='340' side='right'caption='[[6l00]], [[Resolution|resolution]] 1.75Å' scene=''> | <StructureSection load='6l00' size='340' side='right'caption='[[6l00]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6l00]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L00 OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6l00]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_phage_IMEEF1 Enterococcus phage IMEEF1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L00 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l00 OCA], [https://pdbe.org/6l00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l00 RCSB], [https://www.ebi.ac.uk/pdbsum/6l00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l00 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/S5MRN1_9CAUD S5MRN1_9CAUD] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Using bacteriophage-derived endolysins as an alternative strategy for fighting drug-resistant bacteria has recently been garnering renewed interest. However, their application is still hindered by their narrow spectra of activity. In our previous work, we demonstrated that the endolysin LysIME-EF1 possesses efficient bactericidal activity against multiple strains of Enterococcus faecalis (E. faecalis). Herein, we observed an 8 kDa fragment and hypothesized that it contributes to LysIME-EF1 lytic activity. To examine our hypothesis, we determined the structure of LysIME-EF1 at 1.75 A resolution. LysIME-EF1 exhibits a unique architecture in which one full-length LysIME-EF1 forms a tetramer with three additional C-terminal cell-wall binding domains (CBDs) that correspond to the abovementioned 8 kDa fragment. Furthermore, we identified an internal ribosomal binding site (RBS) and alternative start codon within LysIME-EF1 gene, which are demonstrated to be responsible for the translation of the truncated CBD. To elucidate the molecular mechanism for the lytic activity of LysIME-EF1, we combined mutagenesis, lytic activity assays and in vivo animal infection experiments. The results confirmed that the additional LysIME-EF1 CBDs are important for LysIME-EF1 architecture and its lytic activity. To our knowledge, this is the first determined structure of multimeric endolysin encoded by a single gene in E. faecalis phages. As such, it may provide valuable insights into designing potent endolysins against the opportunistic pathogen E. faecalis. | |||
Structural and functional insights into a novel two-component endolysin encoded by a single gene in Enterococcus faecalis phage.,Zhou B, Zhen X, Zhou H, Zhao F, Fan C, Perculija V, Tong Y, Mi Z, Ouyang S PLoS Pathog. 2020 Mar 16;16(3):e1008394. doi: 10.1371/journal.ppat.1008394., eCollection 2020 Mar. PMID:32176738<ref>PMID:32176738</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6l00" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Lysin 3D structures|Lysin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Enterococcus phage IMEEF1]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Ouyang | [[Category: Ouyang SY]] | ||
[[Category: Zhen | [[Category: Zhen XK]] | ||
