5eo7: Difference between revisions

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<StructureSection load='5eo7' size='340' side='right'caption='[[5eo7]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='5eo7' size='340' side='right'caption='[[5eo7]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5eo7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspor Aspor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EO7 FirstGlance]. <br>
<table><tr><td colspan='2'>[[5eo7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_oryzae_RIB40 Aspergillus oryzae RIB40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EO7 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SFU:METHYL+6-DEOXY-1-SELENO-ALPHA-L-GALACTOPYRANOSIDE'>SFU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5eo8|5eo8]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SFU:METHYL+6-DEOXY-1-SELENO-ALPHA-L-GALACTOPYRANOSIDE'>SFU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AO090001000189 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=510516 ASPOR])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eo7 OCA], [https://pdbe.org/5eo7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eo7 RCSB], [https://www.ebi.ac.uk/pdbsum/5eo7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eo7 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5eo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eo7 OCA], [http://pdbe.org/5eo7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5eo7 RCSB], [http://www.ebi.ac.uk/pdbsum/5eo7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5eo7 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/LECF_ASPOR LECF_ASPOR] Lectin that specifically binds to L-fucose and weakly reacts with mannose and N-acetyl-neuraminic acid (PubMed:12092808, PubMed:27318092, PubMed:28041800). Has strongest preference for the alpha-1,6-fucosylated chain (core fucose) on glycoproteins among alpha-1,2-, alpha-1,3-, alpha-1,4-, and alpha-1,6-fucosylated chains (PubMed:17383961, PubMed:19109923). Binds to fucose residues of IgE in mice and human, causing antigen-independent IgE-mediated mast cell activation and anaphylactoid reactions in mice and is possibly implicated in allergic response to Aspergillus oryzae in humans (PubMed:21790704). Induces secretion of pro-inflammatory cytokines IL6 and IL8 implicated in ocular diseases such as mycotic keratitis, probably through its interaction with host toll-like receptors TLR2 and TLR4, followed by up-regulation of pro-inflammatory cytokines (PubMed:28470344).<ref>PMID:12092808</ref> <ref>PMID:17383961</ref> <ref>PMID:19109923</ref> <ref>PMID:21790704</ref> <ref>PMID:27318092</ref> <ref>PMID:28041800</ref> <ref>PMID:28470344</ref>  
The crystal structure of AOL (a fucose-specific lectin of Aspergillus oryzae) has been solved by SAD (single-wavelength anomalous diffraction) and MAD (multi-wavelength anomalous diffraction) phasing of seleno-fucosides. The overall structure is a six-bladed beta-propeller similar to that of other fucose-specific lectins. The fucose moieties of the seleno-fucosides are located in six fucose-binding sites. Although the Arg and Glu/Gln residues bound to the fucose moiety are common to all fucose-binding sites, the amino-acid residues involved in fucose binding at each site are not identical. The varying peak heights of the seleniums in the electron density map suggest that each fucose-binding site has a different carbohydrate binding affinity.
 
Six independent fucose-binding sites in the crystal structure of Aspergillus oryzae lectin.,Makyio H, Shimabukuro J, Suzuki T, Imamura A, Ishida H, Kiso M, Ando H, Kato R Biochem Biophys Res Commun. 2016 Jun 16. pii: S0006-291X(16)30987-1. doi:, 10.1016/j.bbrc.2016.06.069. PMID:27318092<ref>PMID:27318092</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5eo7" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Aspor]]
[[Category: Aspergillus oryzae RIB40]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Ando, H]]
[[Category: Ando H]]
[[Category: Ishida, H]]
[[Category: Ishida H]]
[[Category: Kato, R]]
[[Category: Kato R]]
[[Category: Kiso, M]]
[[Category: Kiso M]]
[[Category: Makyio, H]]
[[Category: Makyio H]]
[[Category: Shimabukuro, S]]
[[Category: Shimabukuro S]]
[[Category: Suzuki, T]]
[[Category: Suzuki T]]
[[Category: Aspergillus oryzae]]
[[Category: Lectin]]
[[Category: Phaing]]
[[Category: Seleno-fucose]]
[[Category: Sugar binding protein]]
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