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New page: left|200px<br /> <applet load="1la6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1la6, resolution 2.0Å" /> '''The crystal structur...
 
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[[Image:1la6.gif|left|200px]]<br />
<applet load="1la6" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1la6, resolution 2.0&Aring;" />
'''The crystal structure of Trematomus newnesi hemoglobin in a partial hemichrome state'''<br />


==Overview==
==The crystal structure of Trematomus newnesi hemoglobin in a partial hemichrome state==
Tetrameric hemoglobins are the most widely used systems in studying, protein cooperativity. Allosteric effects in hemoglobins arise from the, switch between a relaxed (R) state and a tense (T) state occurring upon, oxygen release. Here we report the 2.0-A crystal structure of the main, hemoglobin component of the Antarctic fish Trematomus newnesi, in a, partial hemichrome form. The two alpha-subunit iron atoms are bound to a, CO molecule, whereas in the beta subunits the distal histidine residue is, the sixth ligand of the heme iron. This structure, a tetrameric hemoglobin, in the hemichrome state, demonstrates that the iron coordination by the, distal histidine, usually associated with denaturing states, may be, tolerated in a native-like hemoglobin structure. In addition, several, features of the tertiary and quaternary organization of this structure are, intermediate between the R and T states and agree well with the R --&gt; T, transition state properties obtained by spectroscopic and kinetic, techniques. The analysis of this structure provides a detailed pathway of, heme-heme communication and it indicates that the plasticity of the beta, heme pocket plays a role in the R --&gt; T transition of tetrameric, hemoglobins.
<StructureSection load='1la6' size='340' side='right'caption='[[1la6]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1la6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trematomus_newnesi Trematomus newnesi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LA6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1la6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1la6 OCA], [https://pdbe.org/1la6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1la6 RCSB], [https://www.ebi.ac.uk/pdbsum/1la6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1la6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HBA1_TRENE HBA1_TRENE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/la/1la6_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1la6 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Tetrameric hemoglobins are the most widely used systems in studying protein cooperativity. Allosteric effects in hemoglobins arise from the switch between a relaxed (R) state and a tense (T) state occurring upon oxygen release. Here we report the 2.0-A crystal structure of the main hemoglobin component of the Antarctic fish Trematomus newnesi, in a partial hemichrome form. The two alpha-subunit iron atoms are bound to a CO molecule, whereas in the beta subunits the distal histidine residue is the sixth ligand of the heme iron. This structure, a tetrameric hemoglobin in the hemichrome state, demonstrates that the iron coordination by the distal histidine, usually associated with denaturing states, may be tolerated in a native-like hemoglobin structure. In addition, several features of the tertiary and quaternary organization of this structure are intermediate between the R and T states and agree well with the R --&gt; T transition state properties obtained by spectroscopic and kinetic techniques. The analysis of this structure provides a detailed pathway of heme-heme communication and it indicates that the plasticity of the beta heme pocket plays a role in the R --&gt; T transition of tetrameric hemoglobins.


==About this Structure==
The crystal structure of a tetrameric hemoglobin in a partial hemichrome state.,Riccio A, Vitagliano L, di Prisco G, Zagari A, Mazzarella L Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9801-6. Epub 2002 Jul 1. PMID:12093902<ref>PMID:12093902</ref>
1LA6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Trematomus_newnesi Trematomus newnesi] with ACE, HEM and CMO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1LA6 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
The crystal structure of a tetrameric hemoglobin in a partial hemichrome state., Riccio A, Vitagliano L, di Prisco G, Zagari A, Mazzarella L, Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9801-6. Epub 2002 Jul 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12093902 12093902]
</div>
[[Category: Protein complex]]
<div class="pdbe-citations 1la6" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Trematomus newnesi]]
[[Category: Trematomus newnesi]]
[[Category: Mazzarella, L.]]
[[Category: Mazzarella L]]
[[Category: Prisco, G.di.]]
[[Category: Riccio A]]
[[Category: Riccio, A.]]
[[Category: Vitagliano L]]
[[Category: Vitagliano, L.]]
[[Category: Zagari A]]
[[Category: Zagari, A.]]
[[Category: Di Prisco G]]
[[Category: ACE]]
[[Category: CMO]]
[[Category: HEM]]
[[Category: bishistidine complex]]
[[Category: hemichrome]]
 
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Retrieved from "https://proteopedia.org/1la6"