6w7i: Difference between revisions
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New page: '''Unreleased structure''' The entry 6w7i is ON HOLD Authors: Maheshwari, S., Kim, Y.S., Aripirala, S., Gabelli, S.B. Description: LmFPPS mutant T164Y in complex with 476A, IPP & Ca [[... |
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==LmFPPS mutant T164W in complex with 476A, IPP & Ca== | |||
<StructureSection load='6w7i' size='340' side='right'caption='[[6w7i]], [[Resolution|resolution]] 2.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W7I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W7I FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=476:3-BUTYL-1-(2,2-DIPHOSPHONOETHYL)PYRIDINIUM'>476</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=IPR:ISOPENTYL+PYROPHOSPHATE'>IPR</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w7i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w7i OCA], [https://pdbe.org/6w7i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w7i RCSB], [https://www.ebi.ac.uk/pdbsum/6w7i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w7i ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Farnesyl diphosphate synthase (FPPS) is an isoprenoid chain elongation enzyme that catalyzes the sequential condensation of dimethylallyl diphosphate (C5) with isopentenyl diphosphate (IPP; C5) and the resulting geranyl diphosphate (GPP; C10) with another molecule of IPP, eventually producing farnesyl diphosphate (FPP; C15), which is a precursor for the biosynthesis of a vast majority of isoprenoids. Previous studies of FPPS have highlighted the importance of the structure around the hydrophobic chain elongation path in determining product specificity. To investigate what structural features define the final chain length of the product in FPPS from Leishmania major, we designed and expressed six mutants of LmFPPS by replacing small amino acids around the binding pocket with bulky residues. Using enzymatic assays, binding kinetics, and crystallographic studies, we analyzed the effects of these mutations on the activity and product specificity of FPPS. Our results revealed that replacement of Thr-164 with tryptophan and phenylalanine completely abolished the activity of FPPS. Intriguingly, the T164Y substitution displayed dual product specificity and produced a mixture GPP and FPP as final products, with an activity for FPP synthesis that was lower than that of the wild-type enzyme. These data indicate that Thr-164 is a potential regulator of product specificity. | |||
Identifying Structural Determinants of Product Specificity in Leishmania major Farnesyl Diphosphate Synthase.,Maheshwari S, Kim YS, Aripirala S, Murphy M, Amzel LM, Gabelli SB Biochemistry. 2020 Jul 12. doi: 10.1021/acs.biochem.0c00432. PMID:32584028<ref>PMID:32584028</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Aripirala | <div class="pdbe-citations 6w7i" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Aripirala S]] | |||
[[Category: Gabelli SB]] | |||
[[Category: Kim YS]] | |||
[[Category: Maheshwari S]] | |||
Latest revision as of 11:21, 30 October 2024
LmFPPS mutant T164W in complex with 476A, IPP & Ca
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