5rf0: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: ==PanDDA analysis group deposition -- Crystal Structure of SARS-CoV-2 main protease in complex with POB0073== <StructureSection load='5rf0' size='340' side='right'caption='5rf0, [[Res... |
No edit summary |
||
| (5 intermediate revisions by the same user not shown) | |||
| Line 3: | Line 3: | ||
<StructureSection load='5rf0' size='340' side='right'caption='[[5rf0]], [[Resolution|resolution]] 1.65Å' scene=''> | <StructureSection load='5rf0' size='340' side='right'caption='[[5rf0]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5RF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5RF0 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=T5D:[1-(pyridin-2-yl)cyclopentyl]methanol'>T5D</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=T5D:[1-(pyridin-2-yl)cyclopentyl]methanol'>T5D</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5rf0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5rf0 OCA], [https://pdbe.org/5rf0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5rf0 RCSB], [https://www.ebi.ac.uk/pdbsum/5rf0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5rf0 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
COVID-19, caused by SARS-CoV-2, lacks effective therapeutics. Additionally, no antiviral drugs or vaccines were developed against the closely related coronavirus, SARS-CoV-1 or MERS-CoV, despite previous zoonotic outbreaks. To identify starting points for such therapeutics, we performed a large-scale screen of electrophile and non-covalent fragments through a combined mass spectrometry and X-ray approach against the SARS-CoV-2 main protease, one of two cysteine viral proteases essential for viral replication. Our crystallographic screen identified 71 hits that span the entire active site, as well as 3 hits at the dimer interface. These structures reveal routes to rapidly develop more potent inhibitors through merging of covalent and non-covalent fragment hits; one series of low-reactivity, tractable covalent fragments were progressed to discover improved binders. These combined hits offer unprecedented structural and reactivity information for on-going structure-based drug design against SARS-CoV-2 main protease. | |||
Crystallographic and electrophilic fragment screening of the SARS-CoV-2 main protease.,Douangamath A, Fearon D, Gehrtz P, Krojer T, Lukacik P, Owen CD, Resnick E, Strain-Damerell C, Aimon A, Abranyi-Balogh P, Brandao-Neto J, Carbery A, Davison G, Dias A, Downes TD, Dunnett L, Fairhead M, Firth JD, Jones SP, Keeley A, Keseru GM, Klein HF, Martin MP, Noble MEM, O'Brien P, Powell A, Reddi RN, Skyner R, Snee M, Waring MJ, Wild C, London N, von Delft F, Walsh MA Nat Commun. 2020 Oct 7;11(1):5047. doi: 10.1038/s41467-020-18709-w. PMID:33028810<ref>PMID:33028810</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5rf0" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Aimon | [[Category: Aimon A]] | ||
[[Category: Brandao-Neto | [[Category: Brandao-Neto J]] | ||
[[Category: Carbery | [[Category: Carbery A]] | ||
[[Category: Douangamath A]] | |||
[[Category: Douangamath | [[Category: Dunnett L]] | ||
[[Category: Dunnett | [[Category: Fearon D]] | ||
[[Category: Fearon | [[Category: Gehrtz P]] | ||
[[Category: Gehrtz | [[Category: Krojer T]] | ||
[[Category: Krojer | [[Category: London N]] | ||
[[Category: London | [[Category: Lukacik P]] | ||
[[Category: Lukacik | [[Category: Owen CD]] | ||
[[Category: Owen | [[Category: Powell AJ]] | ||
[[Category: Powell | [[Category: Resnick E]] | ||
[[Category: Resnick | [[Category: Skyner R]] | ||
[[Category: Skyner | [[Category: Snee M]] | ||
[[Category: Snee | [[Category: Strain-Damerell CM]] | ||
[[Category: Strain-Damerell | [[Category: Walsh MA]] | ||
[[Category: Walsh | [[Category: Wild C]] | ||
[[Category: Wild | [[Category: Von Delft F]] | ||
[[Category: | |||
Latest revision as of 13:23, 18 February 2026
PanDDA analysis group deposition -- Crystal Structure of SARS-CoV-2 main protease in complex with POB0073
| ||||||||||||
