6wgi: Difference between revisions

Latest revision as of 14:44, 6 March 2024

Atomic model of the mutant OCCM (ORC-Cdc6-Cdt1-Mcm2-7 with Mcm6 WHD truncation) loaded on DNA at 10.5 A resolution

Structural highlights

6wgi is a 10 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 10Å
Ligands:	AGS, MG
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CDC6_YEAST Plays a crucial role in forming the pre-replicative complexes. Interacts with the origin recognition complex (ORC) and MCM2-7 helicase complex leading to the linking of those complexes and loading of the replicative helicase MCM2-7 onto the pre-replicative complexes. Required for the initiation of DNA replication and then actively participates in the suppression of nuclear division.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7] ^[8] ^[9]

References

↑ Wang B, Feng L, Hu Y, Huang SH, Reynolds CP, Wu L, Jong AY. The essential role of Saccharomyces cerevisiae CDC6 nucleotide-binding site in cell growth, DNA synthesis, and Orc1 association. J Biol Chem. 1999 Mar 19;274(12):8291-8. PMID:10075735
↑ Bueno A, Russell P. Dual functions of CDC6: a yeast protein required for DNA replication also inhibits nuclear division. EMBO J. 1992 Jun;11(6):2167-76. PMID:1600944
↑ Fernandez-Cid A, Riera A, Tognetti S, Herrera MC, Samel S, Evrin C, Winkler C, Gardenal E, Uhle S, Speck C. An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly. Mol Cell. 2013 May 23;50(4):577-88. doi: 10.1016/j.molcel.2013.03.026. Epub 2013 , Apr 18. PMID:23603117 doi:https://dx.doi.org/10.1016/j.molcel.2013.03.026
↑ Piatti S, Lengauer C, Nasmyth K. Cdc6 is an unstable protein whose de novo synthesis in G1 is important for the onset of S phase and for preventing a 'reductional' anaphase in the budding yeast Saccharomyces cerevisiae. EMBO J. 1995 Aug 1;14(15):3788-99. PMID:7641697
↑ Cocker JH, Piatti S, Santocanale C, Nasmyth K, Diffley JF. An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast. Nature. 1996 Jan 11;379(6561):180-2. PMID:8538771 doi:https://dx.doi.org/10.1038/379180a0
↑ Santocanale C, Diffley JF. ORC- and Cdc6-dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae. EMBO J. 1996 Dec 2;15(23):6671-9. PMID:8978693
↑ Detweiler CS, Li JJ. Cdc6p establishes and maintains a state of replication competence during G1 phase. J Cell Sci. 1997 Mar;110 ( Pt 6):753-63. PMID:9099949
↑ Drury LS, Perkins G, Diffley JF. The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast. EMBO J. 1997 Oct 1;16(19):5966-76. PMID:9312054 doi:10.1093/emboj/16.19.5966
↑ Weinreich M, Liang C, Stillman B. The Cdc6p nucleotide-binding motif is required for loading mcm proteins onto chromatin. Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):441-6. PMID:9892652

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OCA

[1] Wang B, Feng L, Hu Y, Huang SH, Reynolds CP, Wu L, Jong AY. The essential role of Saccharomyces cerevisiae CDC6 nucleotide-binding site in cell growth, DNA synthesis, and Orc1 association. J Biol Chem. 1999 Mar 19;274(12):8291-8. PMID:10075735

[2] Bueno A, Russell P. Dual functions of CDC6: a yeast protein required for DNA replication also inhibits nuclear division. EMBO J. 1992 Jun;11(6):2167-76. PMID:1600944

[3] Fernandez-Cid A, Riera A, Tognetti S, Herrera MC, Samel S, Evrin C, Winkler C, Gardenal E, Uhle S, Speck C. An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly. Mol Cell. 2013 May 23;50(4):577-88. doi: 10.1016/j.molcel.2013.03.026. Epub 2013 , Apr 18. PMID:23603117 doi:https://dx.doi.org/10.1016/j.molcel.2013.03.026

[4] Piatti S, Lengauer C, Nasmyth K. Cdc6 is an unstable protein whose de novo synthesis in G1 is important for the onset of S phase and for preventing a 'reductional' anaphase in the budding yeast Saccharomyces cerevisiae. EMBO J. 1995 Aug 1;14(15):3788-99. PMID:7641697

[5] Cocker JH, Piatti S, Santocanale C, Nasmyth K, Diffley JF. An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast. Nature. 1996 Jan 11;379(6561):180-2. PMID:8538771 doi:https://dx.doi.org/10.1038/379180a0

[6] Santocanale C, Diffley JF. ORC- and Cdc6-dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae. EMBO J. 1996 Dec 2;15(23):6671-9. PMID:8978693

[7] Detweiler CS, Li JJ. Cdc6p establishes and maintains a state of replication competence during G1 phase. J Cell Sci. 1997 Mar;110 ( Pt 6):753-63. PMID:9099949

[8] Drury LS, Perkins G, Diffley JF. The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast. EMBO J. 1997 Oct 1;16(19):5966-76. PMID:9312054 doi:10.1093/emboj/16.19.5966

[9] Weinreich M, Liang C, Stillman B. The Cdc6p nucleotide-binding motif is required for loading mcm proteins onto chromatin. Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):441-6. PMID:9892652

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6wgi is ON HOLD  until sometime in the future
+==Atomic model of the mutant OCCM (ORC-Cdc6-Cdt1-Mcm2-7 with Mcm6 WHD truncation) loaded on DNA at 10.5 A resolution==
+<StructureSection load='6wgi' size='340' side='right'caption='[[6wgi]], [[Resolution|resolution]] 10.00&Aring;' scene=''>
-Authors: Yuan, Z., Schneider, S., Dodd, T., Riera, A., Bai, L., Yan, C., Magdalou, I., Ivanov, I., Stillman, B., Li, H., Speck, C.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6wgi]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WGI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WGI FirstGlance]. <br>
-Description: Structural mechanism of helicase loading onto replication origin DNA by ORC-Cdc6
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 10&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-[[Category: Bai, L]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wgi OCA], [https://pdbe.org/6wgi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wgi RCSB], [https://www.ebi.ac.uk/pdbsum/6wgi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wgi ProSAT]</span></td></tr>
-[[Category: Riera, A]]
+</table>
-[[Category: Li, H]]
+== Function ==
-[[Category: Ivanov, I]]
+[https://www.uniprot.org/uniprot/CDC6_YEAST CDC6_YEAST] Plays a crucial role in forming the pre-replicative complexes. Interacts with the origin recognition complex (ORC) and MCM2-7 helicase complex leading to the linking of those complexes and loading of the replicative helicase MCM2-7 onto the pre-replicative complexes. Required for the initiation of DNA replication and then actively participates in the suppression of nuclear division.<ref>PMID:10075735</ref> <ref>PMID:1600944</ref> <ref>PMID:23603117</ref> <ref>PMID:7641697</ref> <ref>PMID:8538771</ref> <ref>PMID:8978693</ref> <ref>PMID:9099949</ref> <ref>PMID:9312054</ref> <ref>PMID:9892652</ref>
-[[Category: Yuan, Z]]
+== References ==
-[[Category: Dodd, T]]
+<references/>
-[[Category: Yan, C]]
+__TOC__
-[[Category: Stillman, B]]
+</StructureSection>
-[[Category: Speck, C]]
+[[Category: Large Structures]]
-[[Category: Schneider, S]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Magdalou, I]]
+[[Category: Bai L]]
+[[Category: Dodd T]]
+[[Category: Ivanov I]]
+[[Category: Li H]]
+[[Category: Magdalou I]]
+[[Category: Riera A]]
+[[Category: Schneider S]]
+[[Category: Speck C]]
+[[Category: Stillman B]]
+[[Category: Yan C]]
+[[Category: Yuan Z]]

6wgi: Difference between revisions

Latest revision as of 14:44, 6 March 2024

Atomic model of the mutant OCCM (ORC-Cdc6-Cdt1-Mcm2-7 with Mcm6 WHD truncation) loaded on DNA at 10.5 A resolution

Structural highlights

Function

References

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