1b3p: Difference between revisions
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==5'-D(*GP*GP*AP*GP*GP*AP*T)-3'== | |||
<StructureSection load='1b3p' size='340' side='right'caption='[[1b3p]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1b3p]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B3P FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b3p OCA], [https://pdbe.org/1b3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b3p RCSB], [https://www.ebi.ac.uk/pdbsum/1b3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b3p ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
== | |||
BACKGROUND: Triplet repeat sequences are of considerable biological importance as the expansion of such tandem arrays can lead to the onset of a range of human diseases. Such sequences can self-pair via mismatch alignments to form higher order structures that have the potential to cause replication blocks, followed by strand slippage and sequence expansion. The all-purine d(GGA)n triplet repeat sequence is of particular interest because purines can align via G.G, A.A and G.A mismatch formation. RESULTS: We have solved the structure of the uniformly 13C,15N-labeled d(G1-G2-A3-G4-G5-A6-T7) sequence in 10 mM Na+ solution. This sequence adopts a novel twofold-symmetric duplex fold where interlocked V-shaped arrowhead motifs are aligned solely via interstrand G1.G4, G2.G5 and A3.A6 mismatch formation. The tip of the arrowhead motif is centered about the p-A3-p step, and symmetry-related local parallel-stranded duplex domains are formed by the G1-G2-A3 and G4-G5-A6 segments of partner strands. CONCLUSIONS: The purine-rich (GGA)n triplet repeat sequence is dispersed throughout the eukaryotic genome. Several features of the arrowhead duplex motif for the (GGA)2 triplet repeat provide a unique scaffold for molecular recognition. These include the large localized bend in the sugar-phosphate backbones, the segmental parallel-stranded alignment of strands and the exposure of the Watson-Crick edges of several mismatched bases. | BACKGROUND: Triplet repeat sequences are of considerable biological importance as the expansion of such tandem arrays can lead to the onset of a range of human diseases. Such sequences can self-pair via mismatch alignments to form higher order structures that have the potential to cause replication blocks, followed by strand slippage and sequence expansion. The all-purine d(GGA)n triplet repeat sequence is of particular interest because purines can align via G.G, A.A and G.A mismatch formation. RESULTS: We have solved the structure of the uniformly 13C,15N-labeled d(G1-G2-A3-G4-G5-A6-T7) sequence in 10 mM Na+ solution. This sequence adopts a novel twofold-symmetric duplex fold where interlocked V-shaped arrowhead motifs are aligned solely via interstrand G1.G4, G2.G5 and A3.A6 mismatch formation. The tip of the arrowhead motif is centered about the p-A3-p step, and symmetry-related local parallel-stranded duplex domains are formed by the G1-G2-A3 and G4-G5-A6 segments of partner strands. CONCLUSIONS: The purine-rich (GGA)n triplet repeat sequence is dispersed throughout the eukaryotic genome. Several features of the arrowhead duplex motif for the (GGA)2 triplet repeat provide a unique scaffold for molecular recognition. These include the large localized bend in the sugar-phosphate backbones, the segmental parallel-stranded alignment of strands and the exposure of the Watson-Crick edges of several mismatched bases. | ||
Interlocked mismatch-aligned arrowhead DNA motifs.,Kettani A, Bouaziz S, Skripkin E, Majumdar A, Wang W, Jones RA, Patel DJ Structure. 1999 Jul 15;7(7):803-15. PMID:10425682<ref>PMID:10425682</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: Bouaziz | <div class="pdbe-citations 1b3p" style="background-color:#fffaf0;"></div> | ||
[[Category: Jones | == References == | ||
[[Category: Kettani | <references/> | ||
[[Category: Majumdar | __TOC__ | ||
[[Category: Patel | </StructureSection> | ||
[[Category: Skripkin | [[Category: Large Structures]] | ||
[[Category: Wang | [[Category: Bouaziz S]] | ||
[[Category: Jones RA]] | |||
[[Category: Kettani A]] | |||
[[Category: Majumdar A]] | |||
[[Category: Patel DJ]] | |||
[[Category: Skripkin E]] | |||
[[Category: Wang W]] | |||
Latest revision as of 11:34, 22 November 2023
5'-D(*GP*GP*AP*GP*GP*AP*T)-3'
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