6ypb: Difference between revisions
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New page: '''Unreleased structure''' The entry 6ypb is ON HOLD Authors: Degut, C., Rety, S., Tisne, C., Baudino, S. Description: NUDIX1 hydrolase from Rosa x hybrida [[Category: Unreleased Struc... |
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The | ==NUDIX1 hydrolase from Rosa x hybrida== | ||
<StructureSection load='6ypb' size='340' side='right'caption='[[6ypb]], [[Resolution|resolution]] 1.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6ypb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rosa_hybrid_cultivar Rosa hybrid cultivar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YPB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YPB FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ypb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ypb OCA], [https://pdbe.org/6ypb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ypb RCSB], [https://www.ebi.ac.uk/pdbsum/6ypb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ypb ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/NUDT1_ROSHC NUDT1_ROSHC] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Roses use a non-canonical pathway involving a Nudix hydrolase, RhNUDX1, to synthesize their monoterpenes, especially geraniol. Here we report the characterization of another expressed NUDX1 gene from the rose cultivar Rosa x wichurana, RwNUDX1-2. In order to study the function of the RwNUDX1-2 protein, we analysed the volatile profiles of an F1 -progeny generated by crossing R. chinensis cv. 'Old Blush' with R. x wichurana. A correlation test of the volatilomes with gene expression data revealed that RwNUDX1-2 is involved in the biosynthesis of a group of sesquiterpenoids: especially E,E-farnesol in addition to other sesquiterpenes. In vitro enzyme assays and heterologous in planta functional characterization of the RwNUDX1-2 gene corroborated this result. A quantitative trait locus (QTL) analysis was performed using the data of E,E-farnesol contents in the progeny and a genetic map constructed based on gene markers. The RwNUDX1-2 gene co-localized with the QTL for E,E-farnesol content, thereby confirming its function in sesquiterpenoid biosynthesis in R. x wichurana. Finally, in order to understand the structural bases for the substrate specificity of rose NUDX proteins, the RhNUDX1 protein was crystallized, and its structure was refined to 1.7 A. By molecular modelling of different rose NUDX1 protein complexes with their respective substrates, a structural basis for substrate discrimination by rose NUDX1 proteins is proposed. | |||
Functional diversification in the Nudix hydrolase gene family drives sesquiterpene biosynthesis in Rosa x wichurana.,Sun P, Degut C, Rety S, Caissard JC, Hibrand-Saint Oyant L, Bony A, Paramita SN, Conart C, Magnard JL, Jeauffre J, Abd-El-Haliem AM, Marie-Magdelaine J, Thouroude T, Baltenweck R, Tisne C, Foucher F, Haring M, Hugueney P, Schuurink RC, Baudino S Plant J. 2020 Jul 8. doi: 10.1111/tpj.14916. PMID:32639596<ref>PMID:32639596</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6ypb" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Rosa hybrid cultivar]] | |||
[[Category: Baudino S]] | |||
[[Category: Degut C]] | |||
[[Category: Rety S]] | |||
[[Category: Tisne C]] | |||
Latest revision as of 13:32, 24 January 2024
NUDIX1 hydrolase from Rosa x hybrida
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