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| ==pH dependent structures of LAH4 in micellar environmnet:mode of acting== | | ==pH dependent structures of LAH4 in micellar environmnet:mode of acting== |
| <StructureSection load='2kjn' size='340' side='right'caption='[[2kjn]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | | <StructureSection load='2kjn' size='340' side='right'caption='[[2kjn]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[2kjn]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KJN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2KJN FirstGlance]. <br> | | <table><tr><td colspan='2'>[[2kjn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KJN FirstGlance]. <br> |
| </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2kjo|2kjo]]</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2kjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kjn OCA], [http://pdbe.org/2kjn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kjn RCSB], [http://www.ebi.ac.uk/pdbsum/2kjn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kjn ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kjn OCA], [https://pdbe.org/2kjn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kjn RCSB], [https://www.ebi.ac.uk/pdbsum/2kjn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kjn ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| The LAH4 family of histidine-rich peptides exhibits potent antimicrobial and DNA transfection activities, both of which require interactions with cellular membranes. The bilayer association of the peptides has been shown to be strongly pH-dependent, with in-planar alignments under acidic conditions and transmembrane orientations when the histidines are discharged. Therefore, we investigated the pH- and temperature-dependent conformations of LAH4 in DPC micellar solutions and in a TFE/PBS solvent mixture. In the presence of detergent and at pH 4.1, LAH4 adopts helical conformations between residues 9 and 24 concomitantly with a high hydrophobic moment. At pH 6.1, a helix-loop-helix structure forms with a hinge encompassing residues His(1)-Ala(1)(3). The data suggest that the high density of histidine residues and the resulting electrostatic repulsion lead to both a decrease in the pK values of the histidines and a less stable alpha-helical conformation of this region. The hinged structure at pH 6.1 facilitates membrane anchoring and insertion. At pH 7.8, the histidines are uncharged and an extended helical conformation including residues 4-21 is again obtained. LAH4 thus exhibits a high degree of conformational plasticity. The structures provide a stroboscopic view of the conformational changes that occur during membrane insertion, and are discussed in the context of antimicrobial activity and DNA transfection.
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| NMR structures of the histidine-rich peptide LAH4 in micellar environments: membrane insertion, pH-dependent mode of antimicrobial action, and DNA transfection.,Georgescu J, Munhoz VH, Bechinger B Biophys J. 2010 Oct 20;99(8):2507-15. PMID:20959091<ref>PMID:20959091</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 2kjn" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Bechinger, B]] | | [[Category: Synthetic construct]] |
| [[Category: Georgescu, J]] | | [[Category: Bechinger B]] |
| [[Category: Amphipathic peptide]] | | [[Category: Georgescu J]] |
| [[Category: De novo protein]]
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