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<StructureSection load='3ixz' size='340' side='right'caption='[[3ixz]], [[Resolution|resolution]] 6.50&Aring;' scene=''>
<StructureSection load='3ixz' size='340' side='right'caption='[[3ixz]], [[Resolution|resolution]] 6.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3ixz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. The October 2009 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sodium-Potassium Pump''  by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2009_10 10.2210/rcsb_pdb/mom_2009_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3IXZ FirstGlance]. <br>
<table><tr><td colspan='2'>[[3ixz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. The October 2009 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Sodium-Potassium Pump''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2009_10 10.2210/rcsb_pdb/mom_2009_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IXZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IXZ FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3b8e|3b8e]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 6.5&#8491;</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrogen/potassium-exchanging_ATPase Hydrogen/potassium-exchanging ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.10 3.6.3.10] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ixz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ixz OCA], [https://pdbe.org/3ixz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ixz RCSB], [https://www.ebi.ac.uk/pdbsum/3ixz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ixz ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3ixz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ixz OCA], [http://pdbe.org/3ixz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ixz RCSB], [http://www.ebi.ac.uk/pdbsum/3ixz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ixz ProSAT]</span></td></tr>
</table>
</table>
== Disease ==
[[http://www.uniprot.org/uniprot/ATP4B_PIG ATP4B_PIG]] Note=Parietal cell autoantigen associated with autoimmune gastritis.
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/ATP4A_PIG ATP4A_PIG]] Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach.  
[https://www.uniprot.org/uniprot/ATP4A_PIG ATP4A_PIG] Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ixz ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ixz ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The gastric H(+),K(+)-ATPase is an ATP-driven proton pump responsible for generating a million-fold proton gradient across the gastric membrane. We present the structure of gastric H(+),K(+)-ATPase at 6.5 A resolution as determined by electron crystallography of two-dimensional crystals. The structure shows the catalytic alpha-subunit and the non-catalytic beta-subunit in a pseudo-E(2)P conformation. Different from Na(+),K(+)-ATPase, the N-terminal tail of the beta-subunit is in direct contact with the phosphorylation domain of the alpha-subunit. This interaction may hold the phosphorylation domain in place, thus stabilizing the enzyme conformation and preventing the reverse reaction of the transport cycle. Indeed, truncation of the beta-subunit N-terminus allowed the reverse reaction to occur. These results suggest that the beta-subunit N-terminus prevents the reverse reaction from E(2)P to E(1)P, which is likely to be relevant for the generation of a large H(+) gradient in vivo situation.
Inter-subunit interaction of gastric H+,K+-ATPase prevents reverse reaction of the transport cycle.,Abe K, Tani K, Nishizawa T, Fujiyoshi Y EMBO J. 2009 Jun 3;28(11):1637-43. Epub 2009 Apr 23. PMID:19387495<ref>PMID:19387495</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3ixz" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[ATPase 3D structures|ATPase 3D structures]]
*[[ATPase 3D structures|ATPase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Hydrogen/potassium-exchanging ATPase]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Sodium-Potassium Pump]]
[[Category: Sodium-Potassium Pump]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
[[Category: Abe, K]]
[[Category: Abe K]]
[[Category: Fujiyoshi, Y]]
[[Category: Fujiyoshi Y]]
[[Category: Nishizawa, T]]
[[Category: Nishizawa T]]
[[Category: Tani, K]]
[[Category: Tani K]]
[[Category: Aluminium fluoride]]
[[Category: Atp-binding]]
[[Category: Disulfide bond]]
[[Category: E2]]
[[Category: Glycoprotein]]
[[Category: H+]]
[[Category: Hydrogen ion transport]]
[[Category: Hydrolase]]
[[Category: Ion pump]]
[[Category: Ion transport]]
[[Category: K+-atpase]]
[[Category: Magnesium]]
[[Category: Membrane]]
[[Category: Membrane protein]]
[[Category: Metal-binding]]
[[Category: Nucleotide-binding]]
[[Category: P-type atpase]]
[[Category: Phosphoprotein]]
[[Category: Potassium]]
[[Category: Potassium transport]]
[[Category: Signal-anchor]]
[[Category: Transmembrane]]
[[Category: Transport]]
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