1b4y: Difference between revisions
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==STRUCTURE AND MECHANISM OF FORMATION OF THE H-Y5 ISOMER OF AN INTRAMOLECULAR DNA TRIPLE HELIX.== | |||
<StructureSection load='1b4y' size='340' side='right'caption='[[1b4y]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1b4y]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B4Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B4Y FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b4y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b4y OCA], [https://pdbe.org/1b4y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b4y RCSB], [https://www.ebi.ac.uk/pdbsum/1b4y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b4y ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
== | |||
H-DNA, thought to play a regulatory role in transcription, exists in two isomeric forms, H-y3 and H-y5. We present the first solution structure of a DNA fragment representing the H-y5 fold. The structure shows the H-y5 triple helix, and for the first time how in an H-DNA isomer the purine strand extension interacts with the triplex loop. It gives direct insight into the mechanism of H-DNA formation, and explains a host of biochemical and biophysical data on the relative stability of the H-DNA isomers. In addition, the observed interaction of the purine strand extension and the triplex loop provides new clues to the design of clamp-type triple helix-forming oligonucleotides. | H-DNA, thought to play a regulatory role in transcription, exists in two isomeric forms, H-y3 and H-y5. We present the first solution structure of a DNA fragment representing the H-y5 fold. The structure shows the H-y5 triple helix, and for the first time how in an H-DNA isomer the purine strand extension interacts with the triplex loop. It gives direct insight into the mechanism of H-DNA formation, and explains a host of biochemical and biophysical data on the relative stability of the H-DNA isomers. In addition, the observed interaction of the purine strand extension and the triplex loop provides new clues to the design of clamp-type triple helix-forming oligonucleotides. | ||
Structure and mechanism of formation of the H-y5 isomer of an intramolecular DNA triple helix.,van Dongen MJ, Doreleijers JF, van der Marel GA, van Boom JH, Hilbers CW, Wijmenga SS Nat Struct Biol. 1999 Sep;6(9):854-9. PMID:10467098<ref>PMID:10467098</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1b4y" style="background-color:#fffaf0;"></div> | |||
[[Category: | == References == | ||
[[Category: Doreleijers | <references/> | ||
[[Category: Hilbers | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Doreleijers JF]] | ||
[[Category: | [[Category: Hilbers CW]] | ||
[[Category: Van Boom JH]] | |||
[[Category: Van Der Marel GA]] | |||
[[Category: Van Dongen MJP]] | |||
[[Category: Wijmenga SS]] | |||
