6ytb: Difference between revisions
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New page: '''Unreleased structure''' The entry 6ytb is ON HOLD Authors: Kuglstatter, A., Leibrock, L., Benz, J. Description: GLYCOSYLATED KNOB/DUMMY-HOLE/DUMMY FC FRAGMENT [[Category: Unreleased... |
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==GLYCOSYLATED KNOB/DUMMY-HOLE/DUMMY FC FRAGMENT== | |||
<StructureSection load='6ytb' size='340' side='right'caption='[[6ytb]], [[Resolution|resolution]] 1.65Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6ytb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YTB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YTB FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ytb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ytb OCA], [https://pdbe.org/6ytb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ytb RCSB], [https://www.ebi.ac.uk/pdbsum/6ytb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ytb ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Generation of bispecific antibodies (bsAbs) requires a combination of compatible binders in formats that support desired functionalities. Here, we report that bsAb-matrices can be generated by Format Chain Exchange (FORCE), enabling screening of combinatorial binder/format spaces. Input molecules for generation of bi/multi-valent bsAbs are monospecific entities similar to knob-into-hole half-antibodies, yet with complementary CH3-interface-modulated and affinity-tagged dummy-chains. These contain mutations that lead to limited interface repulsions without compromising expression or biophysical properties of educts. Mild reduction of combinations of educts triggers spontaneous chain-exchange reactions driven by partially flawed CH3-educt interfaces resolving to perfect complementarity. This generates large bsAb matrices harboring different binders in multiple formats. Benign biophysical properties and good expression yields of educts, combined with simplicity of purification enables process automation. Examples that demonstrate the relevance of screening binder/format combinations are provided as a matrix of bsAbs that simultaneously bind Her1/Her2 and DR5 without encountering binder or format-inflicted interferences. | |||
Format chain exchange (FORCE) for high-throughput generation of bispecific antibodies in combinatorial binder-format matrices.,Dengl S, Mayer K, Bormann F, Duerr H, Hoffmann E, Nussbaum B, Tischler M, Wagner M, Kuglstatter A, Leibrock L, Buldun C, Georges G, Brinkmann U Nat Commun. 2020 Oct 2;11(1):4974. doi: 10.1038/s41467-020-18477-7. PMID:33009381<ref>PMID:33009381</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Benz | <div class="pdbe-citations 6ytb" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Benz J]] | |||
[[Category: Kuglstatter A]] | |||
[[Category: Leibrock L]] | |||
Latest revision as of 09:18, 9 October 2024
GLYCOSYLATED KNOB/DUMMY-HOLE/DUMMY FC FRAGMENT
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