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[[Image:1b8p.gif|left|200px]]
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{{STRUCTURE_1b8p|  PDB=1b8p  |  SCENE=  }}
'''MALATE DEHYDROGENASE FROM AQUASPIRILLUM ARCTICUM'''


==MALATE DEHYDROGENASE FROM AQUASPIRILLUM ARCTICUM==
<StructureSection load='1b8p' size='340' side='right'caption='[[1b8p]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1b8p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquaspirillum_arcticum Aquaspirillum arcticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B8P FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b8p OCA], [https://pdbe.org/1b8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b8p RCSB], [https://www.ebi.ac.uk/pdbsum/1b8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b8p ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MDH_AQUAR MDH_AQUAR]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b8/1b8p_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b8p ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Aquaspillium arcticum is a psychrophilic bacterium that was isolated from arctic sediment and grows optimally at 4 degrees C. We have cloned, purified, and characterized malate dehydrogenase from A. arcticum (Aa MDH). We also have determined the crystal structures of apo-Aa MDH, Aa MDH.NADH binary complex, and Aa MDH.NAD.oxaloacetate ternary complex at 1.9-, 2.1-, and 2.5-A resolutions, respectively. The Aa MDH sequence is most closely related to the sequence of a thermophilic MDH from Thermus flavus (Tf MDH), showing 61% sequence identity and over 90% sequence similarity. Stability studies show that Aa MDH has a half-life of 10 min at 55 degrees C, whereas Tf MDH is fully active at 90 degrees C for 1 h. Aa MDH shows 2-3-fold higher catalytic efficiency compared with a mesophilic or a thermophilic MDH at the temperature range 4-10 degrees C. Structural comparison of Aa MDH and Tf MDH suggests that the increased relative flexibility of active site residues, favorable surface charge distribution for substrate and cofactor, and the reduced intersubunit ion pair interactions may be the major factors for the efficient catalytic activity of Aa MDH at low temperatures.


==Overview==
Structural basis for cold adaptation. Sequence, biochemical properties, and crystal structure of malate dehydrogenase from a psychrophile Aquaspirillium arcticum.,Kim SY, Hwang KY, Kim SH, Sung HC, Han YS, Cho Y J Biol Chem. 1999 Apr 23;274(17):11761-7. PMID:10206992<ref>PMID:10206992</ref>
Aquaspillium arcticum is a psychrophilic bacterium that was isolated from arctic sediment and grows optimally at 4 degrees C. We have cloned, purified, and characterized malate dehydrogenase from A. arcticum (Aa MDH). We also have determined the crystal structures of apo-Aa MDH, Aa MDH.NADH binary complex, and Aa MDH.NAD.oxaloacetate ternary complex at 1.9-, 2.1-, and 2.5-A resolutions, respectively. The Aa MDH sequence is most closely related to the sequence of a thermophilic MDH from Thermus flavus (Tf MDH), showing 61% sequence identity and over 90% sequence similarity. Stability studies show that Aa MDH has a half-life of 10 min at 55 degrees C, whereas Tf MDH is fully active at 90 degrees C for 1 h. Aa MDH shows 2-3-fold higher catalytic efficiency compared with a mesophilic or a thermophilic MDH at the temperature range 4-10 degrees C. Structural comparison of Aa MDH and Tf MDH suggests that the increased relative flexibility of active site residues, favorable surface charge distribution for substrate and cofactor, and the reduced intersubunit ion pair interactions may be the major factors for the efficient catalytic activity of Aa MDH at low temperatures.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1B8P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquaspirillum_arcticum Aquaspirillum arcticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B8P OCA].
</div>
<div class="pdbe-citations 1b8p" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structural basis for cold adaptation. Sequence, biochemical properties, and crystal structure of malate dehydrogenase from a psychrophile Aquaspirillium arcticum., Kim SY, Hwang KY, Kim SH, Sung HC, Han YS, Cho Y, J Biol Chem. 1999 Apr 23;274(17):11761-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10206992 10206992]
*[[Malate Dehydrogenase 3D structures|Malate Dehydrogenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aquaspirillum arcticum]]
[[Category: Aquaspirillum arcticum]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Cho, Y.]]
[[Category: Cho Y]]
[[Category: Han, Y S.]]
[[Category: Han YS]]
[[Category: Hwang, K Y.]]
[[Category: Hwang KY]]
[[Category: Kim, S H.]]
[[Category: Kim S-H]]
[[Category: Kim, S Y.]]
[[Category: Kim SY]]
[[Category: Dehydrogenase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:12:46 2008''

Latest revision as of 23:20, 27 December 2023

MALATE DEHYDROGENASE FROM AQUASPIRILLUM ARCTICUM

1b8p, resolution 1.90Å

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