Sandbox UC 9: Difference between revisions
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===subtitle=== | ===subtitle=== | ||
<ref>PMID:3023912</ref> | |||
<ref>PMID:7120407</ref> | |||
<ref>PMID: 2337600</ref> | |||
<ref>PMID:7308213</ref> | |||
<ref>PMID:17615299</ref> | |||
<Structure load='' size='400' frame='true' align='right' caption='Candida rugosa lipase ([[1trh]], [[1lpm]]).' scene='Lipase_lid_morph/Lightseagreen_hinge/1' /> | |||
For an introduction to the structure and function of lipase, please see the article [[Lipase]]. This ''Lipase lid morph'' article is a supplement to the main article on [[Lipase]]. | |||
''Candida rugosa'' lipase (triacylglycerol hydrolase) has been observed in two conformations, with the "lid" (residues 66-92<ref name='2states1994' />) closed ([[1trh]]) or open ([[1lpm]])<ref name='2states1994'>PMID: 8142901</ref>. | |||
*Closed <font color='lightseagreen'><b>LID</b></font> (<scene name='Lipase_lid_morph/Lightseagreen_hinge/1'>restore initial scene</scene>). | |||
*<scene name='Lipase_lid_morph/Lightseagreen_hinge/4'>Open</scene> <font color='lightseagreen'><b>LID</b></font> with inhibitor (1R)-menthyl hexyl phosphonate (<b><font color='#909090'>C</font> <font color='#ff0d0d'>O</font> <font color='#ff8000'>P</font></b>). | |||
A morph<ref>This is a [[Morphs#Linear_Interpolation|linear interpolation morph]]. The 14-model PDB file is [[Image:Morph-linear-1trh-1lpm.pdb.gz]].</ref> shows the lid opening and closing. | |||
*<scene name='Lipase_lid_morph/Lightseagreen_hinge/2'>Cartoon morph</scene> (<font color='lightseagreen'><b>LID</b></font>). | |||
*<scene name='Lipase_lid_morph/Lightseagreen_hinge/3'>Spacefilling morph</scene> (<font color='lightseagreen'><b>LID</b></font>, <font color='orange'><b>catalytic triad: Ser209, Glu341, and His449</b></font><ref name='2states1994' />).<br> | |||
{{Template:Button Toggle Animation2}} | |||
When the lid is closed, the enzyme surface is largely {{Template:ColorKey_Polar}}. When the lid opens, a {{Template:ColorKey_Hydrophobic}} pocket is exposed with the catalytic triad in the bottom. | |||
*<scene name='Lipase_lid_morph/Lightseagreen_hinge/5'>Spacefilling morph</scene> ({{Template:ColorKey_Polar}}, {{Template:ColorKey_Hydrophobic}}, <font color='orange'><b>catalytic triad: Ser209, Glu341, and His449</b></font><ref name='2states1994' />). | |||
==See Also== | |||
*[[Lipase]], the main article in Proteopedia. | |||
*[[Molecular Playground/Pancreatic Lipase]] | |||
*[http://en.wikipedia.org/wiki/Lipase Lipase in Wikipedia] | |||
==Notes and References== | |||
<references /> | |||
Latest revision as of 19:24, 8 May 2020
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Title
subtitle
[1] [2] [3] [4] [5]
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For an introduction to the structure and function of lipase, please see the article Lipase. This Lipase lid morph article is a supplement to the main article on Lipase.
Candida rugosa lipase (triacylglycerol hydrolase) has been observed in two conformations, with the "lid" (residues 66-92[6]) closed (1trh) or open (1lpm)[6].
- Closed LID (restore initial scene).
- Open LID with inhibitor (1R)-menthyl hexyl phosphonate (C O P).
A morph[7] shows the lid opening and closing.
- Cartoon morph (LID).
- Spacefilling morph (LID, catalytic triad: Ser209, Glu341, and His449[6]).
When the lid is closed, the enzyme surface is largely Polar. When the lid opens, a Hydrophobic pocket is exposed with the catalytic triad in the bottom.
- Spacefilling morph (Polar, Hydrophobic, catalytic triad: Ser209, Glu341, and His449[6]).
See Also
- Lipase, the main article in Proteopedia.
- Molecular Playground/Pancreatic Lipase
- Lipase in Wikipedia
Notes and References
- ↑ Kim KS, Rosenkrantz MS, Guarente L. Saccharomyces cerevisiae contains two functional citrate synthase genes. Mol Cell Biol. 1986 Jun;6(6):1936-42. PMID:3023912
- ↑ Remington S, Wiegand G, Huber R. Crystallographic refinement and atomic models of two different forms of citrate synthase at 2.7 and 1.7 A resolution. J Mol Biol. 1982 Jun 15;158(1):111-52. PMID:7120407
- ↑ Karpusas M, Branchaud B, Remington SJ. Proposed mechanism for the condensation reaction of citrate synthase: 1.9-A structure of the ternary complex with oxaloacetate and carboxymethyl coenzyme A. Biochemistry. 1990 Mar 6;29(9):2213-9. PMID:2337600
- ↑ Bayer E, Bauer B, Eggerer H. Evidence from inhibitor studies for conformational changes of citrate synthase. Eur J Biochem. 1981 Nov;120(1):155-60. PMID:7308213
- ↑ Lee YJ, Hoe KL, Maeng PJ. Yeast cells lacking the CIT1-encoded mitochondrial citrate synthase are hypersusceptible to heat- or aging-induced apoptosis. Mol Biol Cell. 2007 Sep;18(9):3556-67. Epub 2007 Jul 5. PMID:17615299 doi:10.1091/mbc.E07-02-0118
- ↑ 6.0 6.1 6.2 6.3 Grochulski P, Li Y, Schrag JD, Cygler M. Two conformational states of Candida rugosa lipase. Protein Sci. 1994 Jan;3(1):82-91. PMID:8142901
- ↑ This is a linear interpolation morph. The 14-model PDB file is File:Morph-linear-1trh-1lpm.pdb.gz.
