6tzw: Difference between revisions
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==Coiled-coil registry shifts in the F684I mutant of Bicaudal D result in cargo-independent activation of dynein motility== | ==Coiled-coil registry shifts in the F684I mutant of Bicaudal D result in cargo-independent activation of dynein motility== | ||
<StructureSection load='6tzw' size='340' side='right'caption='[[6tzw]]' scene=''> | <StructureSection load='6tzw' size='340' side='right'caption='[[6tzw]], [[Resolution|resolution]] 2.35Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TZW OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6tzw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TZW FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tzw OCA], [https://pdbe.org/6tzw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tzw RCSB], [https://www.ebi.ac.uk/pdbsum/6tzw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tzw ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/BICD_DROME BICD_DROME] This protein is essential for differentiation. It may play a role in localizing of Nanos (a maternal determinant) activity in oocytes. BicD mutations cause Nanos mislocalization and thus bicaudal development. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The dynein adaptor Drosophila Bicaudal D (BicD) is auto-inhibited and activates dynein motility only after cargo is bound, but the underlying mechanism is elusive. In contrast, we show that the full-length BicD/F684I mutant activates dynein processivity even in the absence of cargo. Our X-ray structure of the C-terminal domain of the BicD/F684I mutant reveals a coiled-coil registry shift; in the N-terminal region, the two helices of the homodimer are aligned, whereas they are vertically shifted in the wild-type. One chain is partially disordered and this structural flexibility is confirmed by computations, which reveal that the mutant transitions back and forth between the two registries. We propose that a coiled-coil registry shift upon cargo binding activates BicD for dynein recruitment. Moreover, the human homolog BicD2/F743I exhibits diminished binding of cargo adaptor Nup358, implying that a coiled-coil registry shift may be a mechanism to modulate cargo selection for BicD2-dependent transport pathways. This article is protected by copyright. All rights reserved. | |||
Coiled-coil registry shifts in the F684I mutant of Bicaudal D result in cargo-independent activation of dynein motility.,Cui H, Ali MY, Goyal P, Zhang K, Loh JY, Trybus KM, Solmaz SR Traffic. 2020 May 7. doi: 10.1111/tra.12734. PMID:32378283<ref>PMID:32378283</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6tzw" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Drosophila melanogaster]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cui H]] | [[Category: Cui H]] | ||
[[Category: Solmaz SR]] | [[Category: Solmaz SR]] | ||
Latest revision as of 07:41, 11 October 2023
Coiled-coil registry shifts in the F684I mutant of Bicaudal D result in cargo-independent activation of dynein motility
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