6z1f: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
New page: '''Unreleased structure''' The entry 6z1f is ON HOLD Authors: Description: Category: Unreleased Structures
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
'''Unreleased structure'''


The entry 6z1f is ON HOLD
==CryoEM structure of Rubisco Activase with its substrate Rubisco from Nostoc sp. (strain PCC7120)==
<StructureSection load='6z1f' size='340' side='right'caption='[[6z1f]], [[Resolution|resolution]] 2.86&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6z1f]] is a 22 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z1F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z1F FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.86&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=CAP:2-CARBOXYARABINITOL-1,5-DIPHOSPHATE'>CAP</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z1f OCA], [https://pdbe.org/6z1f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z1f RCSB], [https://www.ebi.ac.uk/pdbsum/6z1f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z1f ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RCA_NOSS1 RCA_NOSS1] Activation of RuBisCO (ribulose-1,5-bisohosphate carboxylase/oxygenase; EC 4.1.1.39) involves the ATP-dependent carboxylation of the epsilon-amino group of lysine leading to a carbamate structure.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Rubisco, the key enzyme of CO(2) fixation in photosynthesis, is prone to inactivation by inhibitory sugar phosphates. Inhibited Rubisco undergoes conformational repair by the hexameric AAA+ chaperone Rubisco activase (Rca) in a process that is not well understood. Here, we performed a structural and mechanistic analysis of cyanobacterial Rca, a close homolog of plant Rca. In the Rca:Rubisco complex, Rca is positioned over the Rubisco catalytic site under repair and pulls the N-terminal tail of the large Rubisco subunit (RbcL) into the hexamer pore. Simultaneous displacement of the C terminus of the adjacent RbcL opens the catalytic site for inhibitor release. An alternative interaction of Rca with Rubisco is mediated by C-terminal domains that resemble the small Rubisco subunit. These domains, together with the N-terminal AAA+ hexamer, ensure that Rca is packaged with Rubisco into carboxysomes. The cyanobacterial Rca is a dual-purpose protein with functions in Rubisco repair and carboxysome organization.


Authors:  
Dual Functions of a Rubisco Activase in Metabolic Repair and Recruitment to Carboxysomes.,Flecken M, Wang H, Popilka L, Hartl FU, Bracher A, Hayer-Hartl M Cell. 2020 Oct 15;183(2):457-473.e20. doi: 10.1016/j.cell.2020.09.010. Epub 2020 , Sep 25. PMID:32979320<ref>PMID:32979320</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 6z1f" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[RuBisCO 3D structures|RuBisCO 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Nostoc sp. PCC 7120 = FACHB-418]]
[[Category: Bracher A]]
[[Category: Flecken M]]
[[Category: Hartl FU]]
[[Category: Hayer-Hartl M]]
[[Category: Popilka L]]
[[Category: Wang H]]
Retrieved from "https://proteopedia.org/6z1f"