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==Solution Structure of the HIV-1 Intron Splicing Silencer and its Interactions with the UP1 Domain of hnRNP A1==
==Solution Structure of the HIV-1 Intron Splicing Silencer and its Interactions with the UP1 Domain of hnRNP A1==
<StructureSection load='2n4l' size='340' side='right'caption='[[2n4l]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
<StructureSection load='2n4l' size='340' side='right'caption='[[2n4l]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2n4l]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N4L OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2N4L FirstGlance]. <br>
<table><tr><td colspan='2'>[[2n4l]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N4L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N4L FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2n4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n4l OCA], [http://pdbe.org/2n4l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2n4l RCSB], [http://www.ebi.ac.uk/pdbsum/2n4l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2n4l ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n4l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n4l OCA], [https://pdbe.org/2n4l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n4l RCSB], [https://www.ebi.ac.uk/pdbsum/2n4l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n4l ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Splicing patterns in human immunodeficiency virus type 1 (HIV-1) are maintained through cis regulatory elements that recruit antagonistic host RNA-binding proteins. The activity of the 3' acceptor site A7 is tightly regulated through a complex network of an intronic splicing silencer (ISS), a bipartite exonic splicing silencer (ESS3a/b), and an exonic splicing enhancer (ESE3). Because HIV-1 splicing depends on protein-RNA interactions, it is important to know the tertiary structures surrounding the splice sites. Herein, we present the NMR solution structure of the phylogenetically conserved ISS stem loop. ISS adopts a stable structure consisting of conserved UG wobble pairs, a folded 2X2 (GU/UA) internal loop, a UU bulge, and a flexible AGUGA apical loop. Calorimetric and biochemical titrations indicate that the UP1 domain of heterogeneous nuclear ribonucleoprotein A1 binds the ISS apical loop site-specifically and with nanomolar affinity. Collectively, this work provides additional insights into how HIV-1 uses a conserved RNA structure to commandeer a host RNA-binding protein.
Solution Structure of the HIV-1 Intron Splicing Silencer and Its Interactions with the UP1 Domain of Heterogeneous Nuclear Ribonucleoprotein (hnRNP) A1.,Jain N, Morgan CE, Rife BD, Salemi M, Tolbert BS J Biol Chem. 2016 Jan 29;291(5):2331-44. doi: 10.1074/jbc.M115.674564. Epub 2015 , Nov 24. PMID:26607354<ref>PMID:26607354</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2n4l" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Jain, N]]
[[Category: Jain N]]
[[Category: Morgan, C E]]
[[Category: Morgan CE]]
[[Category: Rife, B D]]
[[Category: Rife BD]]
[[Category: Salemi, M]]
[[Category: Salemi M]]
[[Category: Tolbert, B S]]
[[Category: Tolbert BS]]
[[Category: Hiv intronic splicing silencer]]
[[Category: Rna]]
[[Category: Rna alternative splicing]]

Latest revision as of 07:03, 1 May 2024

Solution Structure of the HIV-1 Intron Splicing Silencer and its Interactions with the UP1 Domain of hnRNP A1

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