7c7d: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: '''Unreleased structure''' The entry 7c7d is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures |
No edit summary |
||
| (4 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Crystal structure of the catalytic unit of thermostable GH87 alpha-1,3-glucanase from Streptomyces thermodiastaticus strain HF3-3== | |||
<StructureSection load='7c7d' size='340' side='right'caption='[[7c7d]], [[Resolution|resolution]] 1.16Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C7D FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.16Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c7d OCA], [https://pdbe.org/7c7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c7d RCSB], [https://www.ebi.ac.uk/pdbsum/7c7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c7d ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
alpha-1,3-Glucan is a homopolymer composed of D-glucose (Glc) and it is an extracellular polysaccharide found in dental plaque due to Streptococcus species. alpha-1,3-Glucanase from Streptomyces thermodiastaticus strain HF3-3 (Agl-ST) has been identified as a thermostable alpha-1,3-glucanase, which is classified into glycoside hydrolase family 87 (GH87) and specifically hydrolyzes alpha-1,3-glucan with an endo-action. The enzyme has a potential to inhibit the production of dental plaque and to be used for biotechnological applications. Here we show the structure of the catalytic unit of Agl-ST determined at 1.16 A resolution using X-ray crystallography. The catalytic unit is composed of two modules, a beta-sandwich fold module, and a right-handed beta-helix fold module, which resembles other structural characterized GH87 enzymes from Bacillus circulans str. KA-304 and Paenibacillus glycanilyticus str. FH11, with moderate sequence identities between each other (approximately 27% between the catalytic units). However, Agl-ST is smaller in size and more thermally stable than the others. A disulfide bond that anchors the C-terminal coil of the beta-helix fold, which is expected to contribute to thermal stability only exists in the catalytic unit of Agl-ST. | |||
Crystal structure of the catalytic unit of thermostable GH87 alpha-1,3-glucanase from Streptomyces thermodiastaticus strain HF3-3.,Itoh T, Panti N, Hayashi J, Toyotake Y, Matsui D, Yano S, Wakayama M, Hibi T Biochem Biophys Res Commun. 2020 Oct 8. pii: S0006-291X(20)31894-5. doi:, 10.1016/j.bbrc.2020.09.133. PMID:33041007<ref>PMID:33041007</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7c7d" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Hayashi J]] | |||
[[Category: Hibi T]] | |||
[[Category: Itoh T]] | |||
[[Category: Panti N]] | |||
[[Category: Suyotha W]] | |||
[[Category: Toyotake Y]] | |||
[[Category: Wakayama M]] | |||
[[Category: Yano S]] | |||
