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<StructureSection load='6vgw' size='340' side='right'caption='[[6vgw]], [[Resolution|resolution]] 1.51&Aring;' scene=''>
<StructureSection load='6vgw' size='340' side='right'caption='[[6vgw]], [[Resolution|resolution]] 1.51&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6vgw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VGW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VGW FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VGW FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.51&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vgw OCA], [http://pdbe.org/6vgw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vgw RCSB], [http://www.ebi.ac.uk/pdbsum/6vgw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vgw ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vgw OCA], [https://pdbe.org/6vgw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vgw RCSB], [https://www.ebi.ac.uk/pdbsum/6vgw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vgw ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Split inteins are privileged molecular scaffolds for the chemical modification of proteins. Though efficient for in vitro applications, these polypeptide ligases have not been utilized for the semisynthesis of proteins in live cells. Here, we biochemically and structurally characterize the naturally split intein VidaL. We show that this split intein, which features the shortest known N-terminal fragment, supports rapid and efficient protein trans-splicing under a range of conditions, enabling semisynthesis of modified proteins both in vitro and in mammalian cells. The utility of this protein engineering system is illustrated through the traceless assembly of multidomain proteins whose biophysical properties render them incompatible with a single expression system, as well as by the semisynthesis of dual posttranslationally modified histone proteins in live cells. We also exploit the domain swapping function of VidaL to effect simultaneous modification and translocation of the nuclear protein HP1alpha in live cells. Collectively, our studies highlight the VidaL system as a tool for the precise chemical modification of cellular proteins with spatial and temporal control.
Live-cell protein engineering with an ultra-short split intein.,Burton AJ, Haugbro M, Parisi E, Muir TW Proc Natl Acad Sci U S A. 2020 May 18. pii: 2003613117. doi:, 10.1073/pnas.2003613117. PMID:32424098<ref>PMID:32424098</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6vgw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Synthetic construct sequences]]
[[Category: Burton AJ]]
[[Category: Burton, A J]]
[[Category: Haugbro M]]
[[Category: Haugbro, M]]
[[Category: Muir TW]]
[[Category: Muir, T W]]
[[Category: Parisi E]]
[[Category: Parisi, E]]
[[Category: Atypical intein]]
[[Category: Intein]]
[[Category: Protein splicing]]
[[Category: Splicing]]
[[Category: Split intein]]
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