2pwt: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(2 intermediate revisions by the same user not shown)
Line 3: Line 3:
<StructureSection load='2pwt' size='340' side='right'caption='[[2pwt]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='2pwt' size='340' side='right'caption='[[2pwt]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2pwt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PWT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2PWT FirstGlance]. <br>
<table><tr><td colspan='2'>[[2pwt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PWT FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LHA:DOUBLY+FUNCTIONALIZED+PAROMOMYCIN+PM-II-162'>LHA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2pwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pwt OCA], [http://pdbe.org/2pwt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2pwt RCSB], [http://www.ebi.ac.uk/pdbsum/2pwt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2pwt ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LHA:DOUBLY+FUNCTIONALIZED+PAROMOMYCIN+PM-II-162'>LHA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pwt OCA], [https://pdbe.org/2pwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pwt RCSB], [https://www.ebi.ac.uk/pdbsum/2pwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pwt ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of the complex between oligonucleotide containing the bacterial ribosomal decoding site (A site) and the synthetic paromomycin analogue 1, which contains the gamma-amino-alpha-hydroxybutyryl (L-haba) group at position N1 of ring II (2-DOS ring), and an ether chain with an O-phenethylaminoethyl group at position C2'' of ring III, is reported. Interestingly, next to the paromomycin analogue 1 specifically bound to the A site, a second molecule of 1 with a different conformation is observed at the crystal packing interface which mimics the A-minor interaction between two bulged-out adenines from the A site and the codon-anticodon stem of the mRNA-tRNA complex. Improved antibacterial activity supports the conclusion that analogue 1 might affect protein synthesis on the ribosome in two different ways: 1) specific binding to the A site forces maintenance of the "on" state with two bulged out adenines, and 2) a new binding mode of 1 to an A-minor motif which stabilizes complex formation between the ribosome and the mRNA-tRNA complex regardless of whether the codon-anticodon stem is of the cognate or near-cognate type.
Crystal Structure of the Bacterial Ribosomal Decoding Site Complexed with a Synthetic Doubly Functionalized Paromomycin Derivative: a New Specific Binding Mode to an A-Minor Motif Enhances in vitro Antibacterial Activity.,Kondo J, Pachamuthu K, Francois B, Szychowski J, Hanessian S, Westhof E ChemMedChem. 2007 Nov 12;2(11):1631-1638. PMID:17722211<ref>PMID:17722211</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2pwt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Francois, B]]
[[Category: Francois B]]
[[Category: Hanessian, S]]
[[Category: Hanessian S]]
[[Category: Kondo, J]]
[[Category: Kondo J]]
[[Category: Pachamuthu, K]]
[[Category: Pachamuthu K]]
[[Category: Szychowski, J]]
[[Category: Szychowski J]]
[[Category: Westhof, E]]
[[Category: Westhof E]]
[[Category: Aminoglycoside]]
[[Category: Haba group]]
[[Category: Ribosomal decoding site]]
[[Category: Rna]]
[[Category: Rna-antibiotic complex]]
[[Category: X-ray analysis]]

Latest revision as of 13:53, 13 March 2024

Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group

2pwt, resolution 1.80Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2pwt&oldid=4089084"