5ijz

Crystal structure of glutamate dehydrogenase(GDH) from Corynebacterium glutamicum

spinqualitylabelsall models 5ijz, resolution 2.29Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5ijz is a 12 chain structure with sequence from Corynebacterium glutamicum ATCC 13032. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.29Å Ligands: AKG, NAP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function DHE4_CORGL Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. Publication Abstract from PubMed Glutamate dehydrogenase (GDH) is an enzyme involved in the synthesis of amino acids by converting glutamate to alpha-ketoglutarate, and vice versa. To investigate the molecular mechanism of GDH, we determined a crystal structure of the Corynebacterium glutamicum-derived GDH (CgGDH) in complex with its NADP cofactor and alpha-ketoglutarate substrate. CgGDH functions as a hexamer, and each CgGDH monomer comprises 2 separate domains; a Rossmann fold cofactor-binding domain and a substrate-binding domain. The structural comparison between the apo- and cofactor/substrate-binding forms revealed that the CgGDH enzyme undergoes a domain movement during catalysis. In the apo-form, CgGDH exists as an open state, and upon binding of the substrate and cofactor the protein undergoes a conformation change to a closed state. Our structural study also revealed that CgGDH has cofactor specificity for NADP, but not NAD, and this was confirmed by GDH activity measurements. Residues involved in the stabilization of the NADP cofactor and the alpha-ketoglutarate substrate were identified, and their roles in substrate/cofactor binding were confirmed by site-directed mutagenesis experiments. Structural insights into domain movement and cofactor specificity of glutamate dehydrogenase from Corynebacterium glutamicum.,Son HF, Kim IK, Kim KJ Biochem Biophys Res Commun. 2015 Apr 10;459(3):387-92. doi:, 10.1016/j.bbrc.2015.02.109. Epub 2015 Feb 27. PMID:25727019 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Glutamate dehydrogenase 3D structures

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