6z2o: Difference between revisions
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==Crystal structure of wild type OgpA from Akkermansia muciniphila in P 21 21 21== | |||
<StructureSection load='6z2o' size='340' side='right'caption='[[6z2o]], [[Resolution|resolution]] 1.65Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6z2o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Akkermansia_muciniphila_ATCC_BAA-835 Akkermansia muciniphila ATCC BAA-835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z2O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z2O FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.649Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z2o OCA], [https://pdbe.org/6z2o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z2o RCSB], [https://www.ebi.ac.uk/pdbsum/6z2o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z2o ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B2UR60_AKKM8 B2UR60_AKKM8] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Akkermansia muciniphila is a mucin-degrading bacterium commonly found in the human gut that promotes a beneficial effect on health, likely based on the regulation of mucus thickness and gut barrier integrity, but also on the modulation of the immune system. In this work, we focus in OgpA from A. muciniphila, an O-glycopeptidase that exclusively hydrolyzes the peptide bond N-terminal to serine or threonine residues substituted with an O-glycan. We determine the high-resolution X-ray crystal structures of the unliganded form of OgpA, the complex with the glycodrosocin O-glycopeptide substrate and its product, providing a comprehensive set of snapshots of the enzyme along the catalytic cycle. In combination with O-glycopeptide chemistry, enzyme kinetics, and computational methods we unveil the molecular mechanism of O-glycan recognition and specificity for OgpA. The data also contribute to understanding how A. muciniphila processes mucins in the gut, as well as analysis of post-translational O-glycosylation events in proteins. | |||
Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila.,Trastoy B, Naegeli A, Anso I, Sjogren J, Guerin ME Nat Commun. 2020 Sep 24;11(1):4844. doi: 10.1038/s41467-020-18696-y. PMID:32973204<ref>PMID:32973204</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6z2o" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Akkermansia muciniphila ATCC BAA-835]] | |||
[[Category: Large Structures]] | |||
[[Category: Anso I]] | |||
[[Category: Guerin ME]] | |||
[[Category: Naegali A]] | |||
[[Category: Sjogren J]] | |||
[[Category: Trastoy B]] | |||
Latest revision as of 13:40, 24 January 2024
Crystal structure of wild type OgpA from Akkermansia muciniphila in P 21 21 21
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