6za1: Difference between revisions
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New page: '''Unreleased structure''' The entry 6za1 is ON HOLD Authors: Zacarias, S., Temporao, A., Carpentier, P., van der Linden, P., Pereira, I.A.C., Matias, P.M. Description: Structure of [N... |
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The | ==Structure of [NiFeSe] hydrogenase G491A variant from Desulfovibrio vulgaris Hildenborough pressurized with Oxygen gas - structure G491A-O2-hd== | ||
<StructureSection load='6za1' size='340' side='right'caption='[[6za1]], [[Resolution|resolution]] 1.37Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6za1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._Hildenborough Desulfovibrio vulgaris str. Hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZA1 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.37Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=FCO:CARBONMONOXIDE-(DICYANO)+IRON'>FCO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=H2S:HYDROSULFURIC+ACID'>H2S</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6za1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6za1 OCA], [https://pdbe.org/6za1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6za1 RCSB], [https://www.ebi.ac.uk/pdbsum/6za1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6za1 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q72AS4_NITV2 Q72AS4_NITV2] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Hydrogenases are metalloenzymes that catalyse both H2 evolution and uptake. They are gas-processing enzymes with deeply buried active sites, so the gases diffuse through channels that connect the active site to the protein surface. The [NiFeSe] hydrogenases are a special class of hydrogenases containing a selenocysteine as a nickel ligand; they are more catalytically active and less O2-sensitive than standard [NiFe] hydrogenases. Characterisation of the channel system of hydrogenases is important to understand how the inhibitor oxygen reaches the active site to cause oxidative damage. To this end, crystals of Desulfovibrio vulgaris Hildenborough [NiFeSe] hydrogenase were pressurized with krypton and oxygen, and a method for tracking labile O2 molecules was developed, for mapping a hydrophobic channel system similar to that of the [NiFe] enzymes as the major route for gas diffusion. | |||
Exploring the gas access routes in a [NiFeSe] hydrogenase using crystals pressurized with krypton and oxygen.,Zacarias S, Temporao A, Carpentier P, van der Linden P, Pereira IAC, Matias PM J Biol Inorg Chem. 2020 Aug 31. pii: 10.1007/s00775-020-01814-y. doi:, 10.1007/s00775-020-01814-y. PMID:32865640<ref>PMID:32865640</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6za1" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Desulfovibrio vulgaris str. Hildenborough]] | ||
[[Category: Large Structures]] | |||
[[Category: Carpentier P]] | |||
[[Category: Matias PM]] | |||
[[Category: Pereira IAC]] | |||
[[Category: Temporao A]] | |||
[[Category: Zacarias S]] | |||
[[Category: Van der Linden P]] | |||
Latest revision as of 13:23, 17 December 2025
Structure of [NiFeSe] hydrogenase G491A variant from Desulfovibrio vulgaris Hildenborough pressurized with Oxygen gas - structure G491A-O2-hd
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