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| <StructureSection load='5izw' size='340' side='right'caption='[[5izw]], [[Resolution|resolution]] 1.74Å' scene=''> | | <StructureSection load='5izw' size='340' side='right'caption='[[5izw]], [[Resolution|resolution]] 1.74Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[5izw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Miscellaneous_nucleic_acid Miscellaneous nucleic acid]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IZW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5IZW FirstGlance]. <br> | | <table><tr><td colspan='2'>[[5izw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IZW FirstGlance]. <br> |
| </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5izw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5izw OCA], [http://pdbe.org/5izw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5izw RCSB], [http://www.ebi.ac.uk/pdbsum/5izw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5izw ProSAT]</span></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.738Å</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5izw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5izw OCA], [https://pdbe.org/5izw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5izw RCSB], [https://www.ebi.ac.uk/pdbsum/5izw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5izw ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| RNA editing is a post-transcriptional process that modifies the genetic information on RNA molecules. In flowering plants, RNA editing usually alters cytidine to uridine in plastids and mitochondria. The PLS-type pentatricopeptide repeat (PPR) protein and the multiple organellar RNA editing factor (MORF, also known as RNA editing factor interacting protein (RIP)) are two types of key trans-acting factors involved in this process. However, how they cooperate with one another remains unclear. Here, we have characterized the interactions between a designer PLS-type PPR protein (PLS)3PPR and MORF9, and found that RNA-binding activity of (PLS)3PPR is drastically increased on MORF9 binding. We also determined the crystal structures of (PLS)3PPR, MORF9 and the (PLS)3PPR-MORF9 complex. MORF9 binding induces significant compressed conformational changes of (PLS)3PPR, revealing the molecular mechanisms by which MORF9-bound (PLS)3PPR has increased RNA-binding activity. Similarly, increased RNA-binding activity is observed for the natural PLS-type PPR protein, LPA66, in the presence of MORF9. These findings significantly expand our understanding of MORF function in plant organellar RNA editing.
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| MORF9 increases the RNA-binding activity of PLS-type pentatricopeptide repeat protein in plastid RNA editing.,Yan J, Zhang Q, Guan Z, Wang Q, Li L, Ruan F, Lin R, Zou T, Yin P Nat Plants. 2017 Apr 10;3:17037. doi: 10.1038/nplants.2017.37. PMID:28394309<ref>PMID:28394309</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 5izw" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
| [[Category: Miscellaneous nucleic acid]] | | [[Category: Unidentified]] |
| [[Category: Guan, Z]] | | [[Category: Guan Z]] |
| [[Category: Yan, J]] | | [[Category: Yan J]] |
| [[Category: Yin, P]] | | [[Category: Yin P]] |
| [[Category: Zhang, Q]] | | [[Category: Zhang Q]] |
| [[Category: Zou, T]] | | [[Category: Zou T]] |
| [[Category: Complex]]
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| [[Category: Morf]]
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| [[Category: Pentatricopeptide repeat]]
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| [[Category: Rna binding protein]]
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