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[[Image:1bos.gif|left|200px]]
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{{STRUCTURE_1bos|  PDB=1bos  |  SCENE=  }}
'''SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR'''


==SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR==
<StructureSection load='1bos' size='340' side='right'caption='[[1bos]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1bos]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_H-19B Enterobacteria phage H-19B] and [https://en.wikipedia.org/wiki/Phage_h30 Phage h30]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BOS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bos OCA], [https://pdbe.org/1bos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bos RCSB], [https://www.ebi.ac.uk/pdbsum/1bos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bos ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/STXB_BPH19 STXB_BPH19] The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/1bos_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bos ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli strains that cause disease in humans. Like other members of the Shiga toxin family, it consists of an enzymatic (A) subunit and five copies of a binding subunit (the B-pentamer). The B-pentamer binds to a specific glycolipid, globotriaosylceramide (Gb3), on the surface of target cells and thereby plays a crucial role in the entry of the toxin. Here we present the crystal structure at 2.8 A resolution of the SLT-I B-pentamer complexed with an analogue of the Gb3 trisaccharide. The structure reveals a surprising density of binding sites, with three trisaccharide molecules bound to each B-subunit monomer of 69 residues. All 15 trisaccharides bind to one side of the B-pentamer, providing further evidence that this side faces the cell membrane. The structural model is consistent with data from site-directed mutagenesis and binding of carbohydrate analogues, and allows the rational design of therapeutic Gb3 analogues that block the attachment of toxin to cells.


==Overview==
Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its receptor Gb3.,Ling H, Boodhoo A, Hazes B, Cummings MD, Armstrong GD, Brunton JL, Read RJ Biochemistry. 1998 Feb 17;37(7):1777-88. PMID:9485303<ref>PMID:9485303</ref>
Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli strains that cause disease in humans. Like other members of the Shiga toxin family, it consists of an enzymatic (A) subunit and five copies of a binding subunit (the B-pentamer). The B-pentamer binds to a specific glycolipid, globotriaosylceramide (Gb3), on the surface of target cells and thereby plays a crucial role in the entry of the toxin. Here we present the crystal structure at 2.8 A resolution of the SLT-I B-pentamer complexed with an analogue of the Gb3 trisaccharide. The structure reveals a surprising density of binding sites, with three trisaccharide molecules bound to each B-subunit monomer of 69 residues. All 15 trisaccharides bind to one side of the B-pentamer, providing further evidence that this side faces the cell membrane. The structural model is consistent with data from site-directed mutagenesis and binding of carbohydrate analogues, and allows the rational design of therapeutic Gb3 analogues that block the attachment of toxin to cells.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1BOS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_h30,_bacteriophage_h19b Bacteriophage h30, bacteriophage h19b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOS OCA].
</div>
<div class="pdbe-citations 1bos" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its receptor Gb3., Ling H, Boodhoo A, Hazes B, Cummings MD, Armstrong GD, Brunton JL, Read RJ, Biochemistry. 1998 Feb 17;37(7):1777-88. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9485303 9485303]
*[[Shiga toxin 3D structures|Shiga toxin 3D structures]]
[[Category: Bacteriophage h30, bacteriophage h19b]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Armstrong, G D.]]
__TOC__
[[Category: Boodhoo, A.]]
</StructureSection>
[[Category: Brunton, J L.]]
[[Category: Enterobacteria phage H-19B]]
[[Category: Cummings, M D.]]
[[Category: Large Structures]]
[[Category: Hazes, B.]]
[[Category: Phage h30]]
[[Category: Ling, H.]]
[[Category: Armstrong GD]]
[[Category: Read, R J.]]
[[Category: Boodhoo A]]
[[Category: Ob-fold]]
[[Category: Brunton JL]]
[[Category: Protein-carbohydrate recognition]]
[[Category: Cummings MD]]
[[Category: Receptor binding]]
[[Category: Hazes B]]
[[Category: Toxin]]
[[Category: Ling H]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:46:38 2008''
[[Category: Read RJ]]
Retrieved from "https://proteopedia.org/1bos"