7cc3: Difference between revisions
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New page: '''Unreleased structure''' The entry 7cc3 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures |
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==Versatile cis-prenyltransferase MM_0014 from Methanosarcina mazei (crystal type: co-FG)== | |||
<StructureSection load='7cc3' size='340' side='right'caption='[[7cc3]], [[Resolution|resolution]] 1.72Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7cc3]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanosarcina_mazei_Go1 Methanosarcina mazei Go1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CC3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CC3 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.72Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DPO:DIPHOSPHATE'>DPO</scene>, <scene name='pdbligand=FQ0:2-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trienoxy]propane-1,3-diol'>FQ0</scene>, <scene name='pdbligand=FQF:(2R)-3-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trienoxy]propane-1,2-diol'>FQF</scene>, <scene name='pdbligand=FV3:(2S)-3-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trienoxy]propane-1,2-diol'>FV3</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cc3 OCA], [https://pdbe.org/7cc3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cc3 RCSB], [https://www.ebi.ac.uk/pdbsum/7cc3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cc3 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A0F8R9A0_METMZ A0A0F8R9A0_METMZ] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Polyprenyl groups, products of isoprenoid metabolism, are utilized in peptidoglycan biosynthesis, protein N-glycosylation, and other processes. These groups are formed by cis-prenyltransferases, which use allylic prenyl pyrophosphates as prenyl-donors to catalyze the C-prenylation of the general acceptor substrate, isopentenyl pyrophosphate. Repetition of this reaction forms (Z,E-mixed)-polyprenyl pyrophosphates, which are converted later into glycosyl carrier lipids, such as undecaprenyl phosphate and dolichyl phosphate. MM_0014 from the methanogenic archaeon Methanosarcina mazei is known as a versatile cis-prenyltransferase that accepts both isopentenyl pyrophosphate and dimethylallyl pyrophosphate as acceptor substrates. To learn more about this enzyme's catalytic activity, we determined the X-ray crystal structures of MM_0014 in the presence or absence of these substrates. Surprisingly, one structure revealed a complex with O-prenylglycerol, suggesting that the enzyme catalyzed the prenylation of glycerol contained in the crystallization buffer. Further analyses confirmed that the enzyme could catalyze the O-prenylation of small alcohols, such as 2-propanol, expanding our understanding of the catalytic ability of cis-prenyltransferases. | |||
A versatile cis-prenyltransferase from Methanosarcina mazei catalyzes both C- and O-prenylations.,Okada M, Unno H, Emi KI, Matsumoto M, Hemmi H J Biol Chem. 2021 Apr 16:100679. doi: 10.1016/j.jbc.2021.100679. PMID:33872599<ref>PMID:33872599</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7cc3" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Methanosarcina mazei Go1]] | |||
[[Category: Hemmi H]] | |||
[[Category: Unno H]] | |||
Latest revision as of 16:03, 29 November 2023
Versatile cis-prenyltransferase MM_0014 from Methanosarcina mazei (crystal type: co-FG)
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