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New page: left|200px<br /> <applet load="1ggi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ggi, resolution 2.8Å" /> '''CRYSTAL STRUCTURE OF...
 
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[[Image:1ggi.gif|left|200px]]<br />
<applet load="1ggi" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ggi, resolution 2.8&Aring;" />
'''CRYSTAL STRUCTURE OF AN HIV-1 NEUTRALIZING ANTIBODY 50.1 IN COMPLEX WITH ITS V3 LOOP PEPTIDE ANTIGEN'''<br />


==Overview==
==CRYSTAL STRUCTURE OF AN HIV-1 NEUTRALIZING ANTIBODY 50.1 IN COMPLEX WITH ITS V3 LOOP PEPTIDE ANTIGEN==
The crystal structure of the Fab fragment of a human immunodeficiency, virus type 1 (HIV-1) neutralizing monoclonal antibody Fab has been, determined at 2.8 A resolution in complex with a linear 16-residue peptide, from the third hypervariable region (V3) of gp120. The first 9 residues of, the peptide are ordered in the electron density maps, and their, conformation is in partial agreement with the beta-strand-type II, beta-turn structure predicted for this portion of the V3 loop. Notably, several of the peptide residues that are well conserved among different, HIV-1 isolates contact a nonpolar 25-A-long groove in the, antibody-combining site. The largely extended structure of the peptide, differs from the beta-turns seen as the primary determinants in other, published anti-peptide Fab structures. Analysis of the specific, Fab-peptide interactions only partially explains the MN isolate, specificity shown by this antibody.
<StructureSection load='1ggi' size='340' side='right'caption='[[1ggi]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ggi]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GGI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GGI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ggi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ggi OCA], [https://pdbe.org/1ggi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ggi RCSB], [https://www.ebi.ac.uk/pdbsum/1ggi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ggi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GCAA_MOUSE GCAA_MOUSE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gg/1ggi_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ggi ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of the Fab fragment of a human immunodeficiency virus type 1 (HIV-1) neutralizing monoclonal antibody Fab has been determined at 2.8 A resolution in complex with a linear 16-residue peptide from the third hypervariable region (V3) of gp120. The first 9 residues of the peptide are ordered in the electron density maps, and their conformation is in partial agreement with the beta-strand-type II beta-turn structure predicted for this portion of the V3 loop. Notably, several of the peptide residues that are well conserved among different HIV-1 isolates contact a nonpolar 25-A-long groove in the antibody-combining site. The largely extended structure of the peptide differs from the beta-turns seen as the primary determinants in other published anti-peptide Fab structures. Analysis of the specific Fab-peptide interactions only partially explains the MN isolate specificity shown by this antibody.


==About this Structure==
Crystal structure of a human immunodeficiency virus type 1 neutralizing antibody, 50.1, in complex with its V3 loop peptide antigen.,Rini JM, Stanfield RL, Stura EA, Salinas PA, Profy AT, Wilson IA Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6325-9. PMID:8327513<ref>PMID:8327513</ref>
1GGI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GGI OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Crystal structure of a human immunodeficiency virus type 1 neutralizing antibody, 50.1, in complex with its V3 loop peptide antigen., Rini JM, Stanfield RL, Stura EA, Salinas PA, Profy AT, Wilson IA, Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6325-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8327513 8327513]
</div>
[[Category: Protein complex]]
<div class="pdbe-citations 1ggi" style="background-color:#fffaf0;"></div>
[[Category: Rini, J.M.]]
[[Category: Stanfield, R.L.]]
[[Category: Wilson, I.A.]]
[[Category: immunoglobulin]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov  8 14:04:32 2007''
==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Rini JM]]
[[Category: Stanfield RL]]
[[Category: Wilson IA]]
Retrieved from "https://proteopedia.org/1ggi"