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New page: left|200px<br /> <applet load="1ihw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ihw" /> '''SOLUTION STRUCTURE OF THE DNA BINDING DOMAI...
 
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[[Image:1ihw.gif|left|200px]]<br />
<applet load="1ihw" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ihw" />
'''SOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF HIV-1 INTEGRASE, NMR, 40 STRUCTURES'''<br />


==Overview==
==SOLUTION STRUCTURE OF THE DNA BINDING DOMAIN OF HIV-1 INTEGRASE, NMR, 40 STRUCTURES==
The solution structure of the DNA binding domain of HIV-1 integrase, (residues 220-270) has been determined by multidimensional NMR, spectroscopy. The protein is a dimer in solution, and each subunit is, composed of a five-stranded beta-barrel with a topology very similar to, that of the SH3 domain. The dimer is formed by a stacked beta-interface, comprising strands 2, 3, and 4, with the two triple-stranded antiparallel, beta-sheets, one from each subunit, oriented antiparallel to each other., One surface of the dimer, bounded by the loop between strands beta 1 and, beta 2, forms a saddle-shaped groove with dimensions of approximately 24 x, 23 x 12 A in cross section. Lys264, which has been shown from mutational, data to be involved in DNA binding, protrudes from this surface, implicating the saddle-shaped groove as the potential DNA binding site.
<StructureSection load='1ihw' size='340' side='right'caption='[[1ihw]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ihw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IHW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ihw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ihw OCA], [https://pdbe.org/1ihw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ihw RCSB], [https://www.ebi.ac.uk/pdbsum/1ihw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ihw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/POL_HV1Z6 POL_HV1Z6] Integrase performs the integration of the newly synthesized dsDNA copy of the viral genome into the host chromosome. The integrated DNA is called provirus.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The solution structure of the DNA binding domain of HIV-1 integrase (residues 220-270) has been determined by multidimensional NMR spectroscopy. The protein is a dimer in solution, and each subunit is composed of a five-stranded beta-barrel with a topology very similar to that of the SH3 domain. The dimer is formed by a stacked beta-interface comprising strands 2, 3, and 4, with the two triple-stranded antiparallel beta-sheets, one from each subunit, oriented antiparallel to each other. One surface of the dimer, bounded by the loop between strands beta 1 and beta 2, forms a saddle-shaped groove with dimensions of approximately 24 x 23 x 12 A in cross section. Lys264, which has been shown from mutational data to be involved in DNA binding, protrudes from this surface, implicating the saddle-shaped groove as the potential DNA binding site.


==About this Structure==
Solution structure of the DNA binding domain of HIV-1 integrase.,Lodi PJ, Ernst JA, Kuszewski J, Hickman AB, Engelman A, Craigie R, Clore GM, Gronenborn AM Biochemistry. 1995 Aug 8;34(31):9826-33. PMID:7632683<ref>PMID:7632683</ref>
1IHW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus Human immunodeficiency virus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IHW OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Solution structure of the DNA binding domain of HIV-1 integrase., Lodi PJ, Ernst JA, Kuszewski J, Hickman AB, Engelman A, Craigie R, Clore GM, Gronenborn AM, Biochemistry. 1995 Aug 8;34(31):9826-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7632683 7632683]
</div>
[[Category: Human immunodeficiency virus]]
<div class="pdbe-citations 1ihw" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
[[Category: Clore, G.M.]]
[[Category: Ernst, J.A.]]
[[Category: Gronenborn, A.M.]]
[[Category: Lodi, P.J.]]
[[Category: aids]]
[[Category: dna-binding protein]]
[[Category: polyprotein]]


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov  8 14:10:05 2007''
==See Also==
*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human immunodeficiency virus 1]]
[[Category: Large Structures]]
[[Category: Clore GM]]
[[Category: Ernst JA]]
[[Category: Gronenborn AM]]
[[Category: Lodi PJ]]
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