7cjr: Difference between revisions
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==Crystal structure of a periplasmic sensor domain of histidine kinase VbrK== | |||
<StructureSection load='7cjr' size='340' side='right'caption='[[7cjr]], [[Resolution|resolution]] 2.28Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[7cjr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"oceanomonas_parahaemolytica"_(fujino_et_al._1951)_miyamoto_et_al._1961 "oceanomonas parahaemolytica" (fujino et al. 1951) miyamoto et al. 1961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CJR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7CJR FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CGJ74_25135, WR32_19055 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=670 "Oceanomonas parahaemolytica" (Fujino et al. 1951) Miyamoto et al. 1961])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7cjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cjr OCA], [http://pdbe.org/7cjr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7cjr RCSB], [http://www.ebi.ac.uk/pdbsum/7cjr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7cjr ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacterial two-component regulatory systems (TCS) play important roles in sensing environmental stimuli and responding to them by regulating gene expression. VbrK/VbrR, a TCS in Vibrio parahaemolyticus, confers resistance to beta-lactam antibiotics through activating a beta-lactamase gene. Its periplasmic sensor domain was previously suggested to detect beta-lactam antibiotics by direct binding. Here, we report a crystal structure of the periplasmic sensing domain of VbrK (VbrK(SD)) using sulfur-based single-wavelength anomalous diffraction (S-SAD) phasing. Contrary to most bacterial sensor domains which form dimers, we show that VbrK(SD) is a monomer using size exclusion chromatography coupled with multi-angle light scattering. This observation is also supported by molecular dynamics simulations. To quantify the binding affinity of beta-lactam antibiotics to VbrK(SD), we performed isothermal titration calorimetry and other biophysical analyses. Unexpectedly, VbrK(SD) did not show any significant binding to beta-lactam antibiotics. Therefore, we propose that the detection of beta-lactam antibiotics by VbrK is likely to be indirect via an as yet unidentified mechanism. | |||
Crystal structure of the periplasmic sensor domain of histidine kinase VbrK suggests indirect sensing of beta-lactam antibiotics.,Goh BC, Chua YK, Qian X, Lin J, Savko M, Dedon PC, Lescar J J Struct Biol. 2020 Sep 2;212(2):107610. doi: 10.1016/j.jsb.2020.107610. PMID:32890780<ref>PMID:32890780</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 7cjr" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Histidine kinase]] | |||
[[Category: Large Structures]] | |||
[[Category: Chua, Y K]] | |||
[[Category: Goh, B C]] | |||
[[Category: Lescar, J]] | |||
[[Category: Qian, X]] | [[Category: Qian, X]] | ||
[[Category: Savko, M]] | [[Category: Savko, M]] | ||
[[Category: | [[Category: Sensor]] | ||
[[Category: | [[Category: Signaling protein]] | ||
[[Category: | [[Category: Tetratricopeptide repeat]] | ||
[[Category: Transferase]] | |||
Latest revision as of 06:23, 14 October 2020
Crystal structure of a periplasmic sensor domain of histidine kinase VbrK
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