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[[Image:1c8o.gif|left|200px]]
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{{STRUCTURE_1c8o|  PDB=1c8o  |  SCENE=  }}
'''2.9 A STRUCTURE OF CLEAVED VIRAL SERPIN CRMA'''


==2.9 A STRUCTURE OF CLEAVED VIRAL SERPIN CRMA==
<StructureSection load='1c8o' size='340' side='right'caption='[[1c8o]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1c8o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cowpox_virus Cowpox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C8O FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c8o OCA], [https://pdbe.org/1c8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c8o RCSB], [https://www.ebi.ac.uk/pdbsum/1c8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c8o ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPI2_CWPXB SPI2_CWPXB] Specific and potent inhibitor of the interleukin-1-beta converting enzyme (ICE) thereby suppressing an interleukin-1 beta response to infection. The inhibition of ICE by crmA is an example of a "cross-class" interaction, in which a serpin inhibits a non-serine proteinase. Also inhibits granzyme B.<ref>PMID:1339309</ref> <ref>PMID:8034697</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c8/1c8o_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c8o ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CrmA is an unusual viral serpin that inhibits both cysteine and serine proteinases involved in the regulation of host inflammatory and apoptosis processes. It differs from other members of the serpin superfamily by having a reactive center loop that is one residue shorter, and by its apparent inability to form SDS-stable covalent complexes with cysteine proteinases. To obtain insight into the inhibitory mechanism of crmA, we determined the crystal structure of reactive center loop-cleaved crmA to 2.9 A resolution. The structure, which is the first of a viral serpin, suggests that crmA can inhibit cysteine proteinases by a mechanism analogous to that used by other serpins against serine proteinases. However, one striking difference from other serpins, which may be significant for in vivo function, is an additional highly charged antiparallel strand for b sheet A, whose sequence and length are unique to crmA.


==Overview==
Crystal structure of viral serpin crmA provides insights into its mechanism of cysteine proteinase inhibition.,Simonovic M, Gettins PGW, Volz K Protein Sci. 2000 Aug;9(8):1423-7. PMID:10975564<ref>PMID:10975564</ref>
CrmA is an unusual viral serpin that inhibits both cysteine and serine proteinases involved in the regulation of host inflammatory and apoptosis processes. It differs from other members of the serpin superfamily by having a reactive center loop that is one residue shorter, and by its apparent inability to form SDS-stable covalent complexes with cysteine proteinases. To obtain insight into the inhibitory mechanism of crmA, we determined the crystal structure of reactive center loop-cleaved crmA to 2.9 A resolution. The structure, which is the first of a viral serpin, suggests that crmA can inhibit cysteine proteinases by a mechanism analogous to that used by other serpins against serine proteinases. However, one striking difference from other serpins, which may be significant for in vivo function, is an additional highly charged antiparallel strand for b sheet A, whose sequence and length are unique to crmA.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
1C8O is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Cowpox_virus Cowpox virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C8O OCA].
</div>
<div class="pdbe-citations 1c8o" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Crystal structure of viral serpin crmA provides insights into its mechanism of cysteine proteinase inhibition., Simonovic M, Gettins PGW, Volz K, Protein Sci. 2000 Aug;9(8):1423-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10975564 10975564]
*[[Serpin 3D structures|Serpin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cowpox virus]]
[[Category: Cowpox virus]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Gettins, P G.W.]]
[[Category: Gettins PGW]]
[[Category: Simonovic, M.]]
[[Category: Simonovic M]]
[[Category: Volz, K.]]
[[Category: Volz K]]
[[Category: Serpin fold]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 12:27:57 2008''

Latest revision as of 23:25, 27 December 2023

2.9 A STRUCTURE OF CLEAVED VIRAL SERPIN CRMA

1c8o, resolution 2.90Å

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