7jmu: Difference between revisions
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==Hen egg-white lysozyme with ionic liquid ethylammonium nitrate== | |||
<StructureSection load='7jmu' size='340' side='right'caption='[[7jmu]], [[Resolution|resolution]] 1.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JMU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JMU FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jmu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jmu OCA], [https://pdbe.org/7jmu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jmu RCSB], [https://www.ebi.ac.uk/pdbsum/7jmu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jmu ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Solvents that support protein functionality are important for biochemical applications, and new solvents are required. Here we employ FTIR and fluorescence spectroscopies, small angle X-ray scattering (SAXS) and X-ray crystallography to understand conformational changes of lysozyme with ionic liquids (ILs) added. Spectroscopic techniques identified that the secondary structure of lysozyme was maintained at the lower IL concentrations of 1 and 5 mol%, though the Tryptophan environment was significantly altered with nitrate-based ILs present. SAXS experiments indicated that the radius of gyration of lysozyme increased with 1 mol% IL present, and then decreased with increasing IL concentrations. The tertiary structure, particularly the loop regions, changed as a function of IL concentration, and this depended on the IL type. The crystallographic structure of lysozyme with the IL of ethylammonium nitrate present confirmed the loop region was extended, and identified three specific binding sites with nitrate ions, and that the positively charged areas were IL sensitive regions. This work provides a detailed understanding of lysozyme conformational changes in the presence of ILs. This approach can be extended to other functionally-important proteins. | |||
Lysozyme conformational changes with ionic liquids: Spectroscopic, small angle x-ray scattering and crystallographic study.,Han Q, Smith KM, Darmanin C, Ryan TM, Drummond CJ, Greaves TL J Colloid Interface Sci. 2020 Oct 13. pii: S0021-9797(20)31347-3. doi:, 10.1016/j.jcis.2020.10.024. PMID:33109332<ref>PMID:33109332</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Darmanin | <div class="pdbe-citations 7jmu" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Lysozyme 3D structures|Lysozyme 3D structures]] | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Darmanin C]] | |||
[[Category: Drummond C]] | |||
[[Category: Greaves T]] | |||
[[Category: Han Q]] | |||
[[Category: Smith K]] | |||
Latest revision as of 11:30, 30 October 2024
Hen egg-white lysozyme with ionic liquid ethylammonium nitrate
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