6m0d: Difference between revisions

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<StructureSection load='6m0d' size='340' side='right'caption='[[6m0d]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='6m0d' size='340' side='right'caption='[[6m0d]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6m0d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Beii9 Beii9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M0D OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M0D FirstGlance]. <br>
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M0D FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Bind_2021 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=395963 BEII9])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Levansucrase Levansucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m0d OCA], [https://pdbe.org/6m0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m0d RCSB], [https://www.ebi.ac.uk/pdbsum/6m0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m0d ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m0d OCA], [http://pdbe.org/6m0d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m0d RCSB], [http://www.ebi.ac.uk/pdbsum/6m0d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m0d ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
An enzyme belonging to glycoside hydrolase family 68 (GH68) from Beijerinckia indica subsp. indica NBRC 3744 was expressed in Escherichia coli. Biochemical characterization showed that the enzyme was identified to be a beta-fructosyltransferase (BiBftA). Crystallization of a full-length BiBftA was initially attempted, but no crystals were obtained. We constructed a variant in which 5 residues (Pro199-Gly203) and 13 residues (Leu522-Gln534) in potentially flexible regions were deleted, and we successfully crystallized this variant BiBftA. BiBftA is composed of a five-bladed beta-propeller fold as in other GH68 enzymes. The structure of BiBftA in complex with fructose unexpectedly indicated that one beta-fructofuranose (beta-Fruf) molecule and one beta-fructopyranose molecule bind to the catalytic pocket. The orientation of beta-Fruf at subsite -1 is tilted from the orientation observed in most GH68 enzymes, presenting a second structure of a GH68 enzyme in complex with the tilted binding mode of beta-Fruf.
Crystal structure of a glycoside hydrolase family 68 beta-fructosyltransferase from Beijerinckia indica subsp. indica in complex with fructose.,Tonozuka T, Kitamura J, Nagaya M, Kawai R, Nishikawa A, Hirano K, Tamura K, Fujii T, Tochio T Biosci Biotechnol Biochem. 2020 Aug 4:1-13. doi: 10.1080/09168451.2020.1804317. PMID:32752982<ref>PMID:32752982</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6m0d" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Beii9]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Levansucrase]]
[[Category: Tonozuka T]]
[[Category: Tonozuka, T]]
[[Category: Fructooligosaccharide]]
[[Category: Gh68]]
[[Category: Hydrolase]]
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